Assessment of genetic diversity among wheat selectedegenotypese and local varieties for saltetolerance by usinge RAPD and ISSR analysis

Other Title(s)

تقدير التباين الوراثي بين التراكيب الوراثية المنتخبة و الأصناف المحمية لصفة تحمل المموحة باستخدام تحاليل RAPD و ISSR

Joint Authors

Ismail, Iman Numan
Majid, Duha Muyassar
al-Mashhadani, Ibrahim Ismail
Sakran, Nur Muhammad

Source

Iraqi Journal of Science

Issue

Vol. 59, Issue 1B (31 Mar. 2018), pp.278-286, 9 p.

Publisher

University of Baghdad College of Science

Publication Date

2018-03-31

Country of Publication

Iraq

No. of Pages

9

Main Subjects

Botany

Topics

Abstract EN

Three genotypes of wheat developede for saltetoleranceethroughe plant breeding program and two local cultivars were screened for genetic variation under salinity conditions through RAPD and ISSR markers.

Eight selected primers (OP1-06, OPE-16, OPN-07, OPO-17, OPD-20, OPL-05, OPI-01 and OPJ-13) were used in randomeamplifiede polymorphic eDNA (RAPD-PCR reaction) and three selected primers (UBC 809, UBC 810 and UBC 811) were used in ISSR markers.

According to the results of the amplification and ISSR markers, the genetic distance and dendogram illustratedegeneticefingerprint and relationshipsebetweeneselected genotypes and local cultivars were determinant.

Results revealed that there are genetics differences between the selected genotypes and the local cultivars in some specific segment at different size (bp) with all primers which used in this study, except the result of the primer (OPE-16) showed that there are no bands appeared in all selected genotypes and local cultivars.

Other results of RAPD markers showed that there are differences among the selected genotypes in their banding patterns only with primers (OP1-06, OPN-07 and OPO-17) at different size.

The results of ISSR markers showed differences that there are also differences between the selected genotypes and local cultivars in specific segment with the three primers which used, the selected genotypes were similar in banding patterns with UBC 809 and UBC 811primers.

Genetically, the results showed that all the selected genotypes and local cultivars differed in their genetic distance, variations among the selected genotypes in their genetic distance.

In conclusion the selected genotypes (salt tolerant) genetically differed from the local cultivars (salt sensitive

American Psychological Association (APA)

Majid, Duha Muyassar& Ismail, Iman Numan& al-Mashhadani, Ibrahim Ismail& Sakran, Nur Muhammad. 2018. Assessment of genetic diversity among wheat selectedegenotypese and local varieties for saltetolerance by usinge RAPD and ISSR analysis. Iraqi Journal of Science،Vol. 59, no. 1B, pp.278-286.
https://search.emarefa.net/detail/BIM-832073

Modern Language Association (MLA)

Majid, Duha Muyassar…[et al.]. Assessment of genetic diversity among wheat selectedegenotypese and local varieties for saltetolerance by usinge RAPD and ISSR analysis. Iraqi Journal of Science Vol. 59, no. 1B (2018), pp.278-286.
https://search.emarefa.net/detail/BIM-832073

American Medical Association (AMA)

Majid, Duha Muyassar& Ismail, Iman Numan& al-Mashhadani, Ibrahim Ismail& Sakran, Nur Muhammad. Assessment of genetic diversity among wheat selectedegenotypese and local varieties for saltetolerance by usinge RAPD and ISSR analysis. Iraqi Journal of Science. 2018. Vol. 59, no. 1B, pp.278-286.
https://search.emarefa.net/detail/BIM-832073

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references : p. 285-286

Record ID

BIM-832073