Comparison between antitumor activity of live-attenuated measles virus and cisplatin on ki-67 expression of colon cancer cell line (SW‑480)‎ in vitro

Abstract EN

Background : Previous studies declare that Ki-67 protein (Ki-67P) expression was shown to be correlated with cell proliferation and was considered as a prognostic marker for different cancer diseases.

Objective: The aim of this study was to investigate the relation between Ki-67 and the cytotoxic effect of MV and cisplatin chemotherapy in inhibition of the proliferation of human colon cancer cell line.

Materials and Methods : Colon cancer cell line (SW-480) was cultured in microtiter plates incubated in the presence of different titers of measles virus and different concentrations of cisplatin.

The expression titer of Human Ki-67 protein was determined in cell culture supernatant by quantitative ELISA.

The optical density (OD) was measured with spectrophotometry at a wavelength of 450 nm.

The OD value is proportional to the concentration of human Ki-67P.

The concentration of human Ki-67P in samples was calculated by comparing the OD of the samples with the standard curve.

Results: The expression of Ki-67P was decreased in relation to the virus titer and concentration of cisplatin in treated cell groups.

The results showed significant differences in the level of cellular expression of protein (Ki-67) between the virus-infected cells, cisplatin-treated, and the l untreated control cells group (P ≤ 0.001).

The lowest Ki-67P expression was recorded after treatment of SW-480 colon tumor cell with high concentration of measles virus.

Conclusion: Both live-attenuated MV (Edmonton strain) and cisplatin could reduce Ki-67P expression in tumor cell line when treated with either one of them.

Therefore, they have beneficial effects in reducing the resistance to chemotherapy and radiotherapy.