Comparative evaluation of extraction methods for total proteins from crocus sativus L. (Saffron)‎

Joint Authors

Mehraj, Sayyid Sana
Kamili, Azra N.
Nadhir, Ruqayah
Haq, Ihtisham
Balkhi, Henah Mehraj

Source

Saudi Journal of Biological Sciences

Issue

Vol. 25, Issue 8 (31 Dec. 2018), pp.1603-1608, 6 p.

Publisher

Saudi Biological Society

Publication Date

2018-12-31

Country of Publication

Saudi Arabia

No. of Pages

6

Main Topic

Biology

Topics

Abstract EN

Broadly speaking proteomic studies are one of the various techniques of utmost importance for understanding complex biological processes that occur under inductive conditions and revealing the multidimensional aspects of Crocus sativus in biological systems. In order to get an insight into the molecular changes and to characterize the variations in protein expression of C. sativus, a detailed proteomic analysis on one-dimensional gel electrophoresis is one of the basic steps to accomplish. We have compared total protein profiles of C. sativus extracted by three different recipes and analyzed on 10% sodium dodecyl sulfate polyacrylamide gels. Gels were subjected to densitometric analysis for further characterization. Among three different protocols NP-40 extraction buffer recipe resulted in the extraction of proteins most efficiently with minimum background and streaking. There was maximum solubilization of proteins with high efficiency. Such a profile can be used for high precision analysis of differential protein expression. This work is an attempt to assist researchers in effective extraction of proteins from C. sativus. As a researcher faces a perplexing array of choices as where to start we describe a method based on our collective analysis of the different protein protocols. This paper presents a method that could be applied at the outset of any proteomic study.

American Psychological Association (APA)

Kamili, Azra N.& Nadhir, Ruqayah& Haq, Ihtisham& Balkhi, Henah Mehraj& Mehraj, Sayyid Sana. 2018. Comparative evaluation of extraction methods for total proteins from crocus sativus L. (Saffron). Saudi Journal of Biological Sciences،Vol. 25, no. 8, pp.1603-1608.
https://search.emarefa.net/detail/BIM-832877

Modern Language Association (MLA)

Mehraj, Sayyid Sana…[et al.]. Comparative evaluation of extraction methods for total proteins from crocus sativus L. (Saffron). Saudi Journal of Biological Sciences Vol. 25, no. 8 (Dec. 2018), pp.1603-1608.
https://search.emarefa.net/detail/BIM-832877

American Medical Association (AMA)

Kamili, Azra N.& Nadhir, Ruqayah& Haq, Ihtisham& Balkhi, Henah Mehraj& Mehraj, Sayyid Sana. Comparative evaluation of extraction methods for total proteins from crocus sativus L. (Saffron). Saudi Journal of Biological Sciences. 2018. Vol. 25, no. 8, pp.1603-1608.
https://search.emarefa.net/detail/BIM-832877

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references : p. 1608

Record ID

BIM-832877