The effect of soluble beta glucan of saccharomyces cerevisiae on growth of amn3 tumor cell line in vivo

الملخص EN

Background and objectives This study was designed to evaluate the anticancer effects of the soluble beta glucan (ß-glucan) local and commercial extracts of the Saccharomyces cerevisiae on cancer cell line AMN3 in vivo.

Methods :In vivo study was performed on cancer cell line murine mammary adenocarcinoma (AMN-3) cell line and the median lethal dose (LD50) of β-glucan extract through SC administration in BALB/c mice.

Results: BALB/c mice showed no LD50 to all the concentration of ß-glucan (200, 400, 600, 800, 1000 μg/ml).The effect of the ß-glucan extract on relative tumor volume (RTV) for three weeks (that treated after 24hrs of tumor implantation ) , and also for one month (after tumor implantation) had the best effect when compared with commercial extract.

RTV was not denoted in mice groups that treated before tumor cell implantation and gave the best protocol for cancer treatment.

The period of three weeks show that all groups have significant inhibition (P<0.05) percentage (38.6,42.6,50.14, and 51.67)% for local extract, (35.22, 42.17, 43.5 and 45.5)% for commercial , so the local and commercial extracts gradually increase the tumour growth inhibition (TGI) depends to the time, except in commercial group showed decrease in TGI (38.3%) at fifteenth day ,also the local extract showed decrease in TGI at the periods of sixth (35.8%) days, reveals tumour growth inhibition of local and commercial extracts for one month .

Conclusions: The soluble β-glucan of Saccharomyces cerevisiae (local & commercial extracts) had a great potent cytotoxic effect against tumor cells line AMN-3 in mice.