Cytotoxic effects of two extracts from garlic (Allium sativum L.)‎ cultivars on the human squamous carcinoma cell line SCC-15

Source

Saudi Journal of Biological Sciences

Issue

Vol. 25, Issue 8 (31 Dec. 2018), pp.1703-1712, 10 p.

Publisher

Saudi Biological Society

Publication Date

2018-12-31

Country of Publication

Saudi Arabia

No. of Pages

10

Main Subjects

Biology

Topics

Abstract EN

Garlic (Allium sativum L., Alliaceae) has acquired a reputation as a therapeutic agent and herbal remedy to prevent and treat several pathologies.

The aim of the present study was to determine the effect of two Allium sativum L.

cultivars, Harnas´ and Morado, on reactive oxygen species (ROS) production, viability and apoptotic process in human squamous carcinoma cell line SCC-15.

The experiments were conducted on SCC-15 cell line exposed to increasing concentrations of garlic extracts of 0.062, 0.125, 0.250, 0.500 and 1.000 mg/mL.

After the experiments, ROS formation, caspase-3 activity and neutral red uptake were measured in the cells, and in a collected medium lactate dehydrogenase (LDH) release was measured.

The Spanish cultivar Morado has demonstrated higher potential to stimulate ROS production in SCC-15 cells after a short time period (6 h) than the Polish cultivar Harnas´.

However, the Polish cultivar Harnas´ manifested more prolonged potential to stimulate ROS production in SCC-15 cells.

Both studied garlic extracts induced cytotoxicity on SCC-15 cell line which was probably ROS-dependent.

We also determined that in SCC-15 cells high concentrations of studied extracts did not cause activation of caspase-3 which suggested caspase-independent or necrotic cell death.

American Psychological Association (APA)

Szychowski, Konrad A.& Binduga, Urszula E.& Tkaczyk, Kamila Rybczyn ska& Leja, Marcin L.& Gminski, Jan. 2018. Cytotoxic effects of two extracts from garlic (Allium sativum L.) cultivars on the human squamous carcinoma cell line SCC-15. Saudi Journal of Biological Sciences،Vol. 25, no. 8, pp.1703-1712.
https://search.emarefa.net/detail/BIM-835024

Modern Language Association (MLA)

Szychowski, Konrad A.…[et al.]. Cytotoxic effects of two extracts from garlic (Allium sativum L.) cultivars on the human squamous carcinoma cell line SCC-15. Saudi Journal of Biological Sciences Vol. 25, no. 8 (Dec. 2018), pp.1703-1712.
https://search.emarefa.net/detail/BIM-835024

American Medical Association (AMA)

Szychowski, Konrad A.& Binduga, Urszula E.& Tkaczyk, Kamila Rybczyn ska& Leja, Marcin L.& Gminski, Jan. Cytotoxic effects of two extracts from garlic (Allium sativum L.) cultivars on the human squamous carcinoma cell line SCC-15. Saudi Journal of Biological Sciences. 2018. Vol. 25, no. 8, pp.1703-1712.
https://search.emarefa.net/detail/BIM-835024

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references : p. 1711-1712

Record ID

BIM-835024