Triterpenoid saponins investigation and pharmacological (Cytotoxic and Antioxidant) properties of Bacopa monnieri L. cultivated in Iraq
Other Title(s)
الاستقصاء عن الصابونيات ثلاثية التربين و خصائصها الدوائية (السمية الخلوية، و مضاد للأكسدة) في نبات ال bacopa monnieri L المزروع في العراق
Author
Source
Issue
Vol. 15, Issue 2 (30 Jun. 2018), pp.123-129, 7 p.
Publisher
University of Baghdad College of Science for Women
Publication Date
2018-06-30
Country of Publication
Iraq
No. of Pages
7
Main Subjects
Abstract EN
Bacopa monnieri L.
(Scrophulariaceae), synoname is Herpestis monniera that provides bioactive compounds, especially triterpenoid saponins (Bacosides) which exhibits an important biological activities, like hypothyroidism, anticonvulsant, memory enhancing and antistress.
Because there are no researches about B.
monnieri L.
plant that grow in Iraq, and there active compounds especially triterpenoid saponin (TS), and there effects.
This study was detected the presence of (TS) in, and examined the cytotoxic and the antioxidant activity of these compounds in vitro.
The study was included the extraction and identification of TS from the whole parts of B.
monnieri L.
by using three methods, and the best yield was analyzed by High Performance Liquid Chromatographic (HPLC) to identify bacosides compounds.
In vitro, TS was examined the cytotoxic activity against two cancer cell lines, human cervical cancer (Hela), rhabdomyo sarcoma (RD), and rat embryogenic fibroblast (REF) as a normal cell, at concentrations of 62.5, 125, 250, 500 and 1000 μg/ml at 24, 48, and 72-hr.
Free radical 1,1Dyphenyl-2-picrylhydrazyl radical (DPPH) was used for testing the ability of the TS as antioxidant at 20, 40, 60, 80, 100 μg/ml concentrations.
The results revealed that B.
monnieri plant had many components of bacosides when detected by HPLC.
Cytotoxic study suggested that TS inhibited the growth of cancer cells, and this effect is depending on the extraction of TS concentration.
The effect of TS on Hela cell line was more than that for RD, while the highest effect of TS on Hela was at the concentration of 250 μg/ml after 48 hr.
The cytotoxic effect has a significant effect at (P≤0.05).
The results revealed that TS has high antioxidant influence 99.37% at 100 μg/ml concentrations, followed by 98.20% in 80 μg/ml concentration.
American Psychological Association (APA)
Allush, Bushra Muhammad Jabir. 2018. Triterpenoid saponins investigation and pharmacological (Cytotoxic and Antioxidant) properties of Bacopa monnieri L. cultivated in Iraq. Baghdad Science Journal،Vol. 15, no. 2, pp.123-129.
https://search.emarefa.net/detail/BIM-837417
Modern Language Association (MLA)
Allush, Bushra Muhammad Jabir. Triterpenoid saponins investigation and pharmacological (Cytotoxic and Antioxidant) properties of Bacopa monnieri L. cultivated in Iraq. Baghdad Science Journal Vol. 15, no. 2 (2018), pp.123-129.
https://search.emarefa.net/detail/BIM-837417
American Medical Association (AMA)
Allush, Bushra Muhammad Jabir. Triterpenoid saponins investigation and pharmacological (Cytotoxic and Antioxidant) properties of Bacopa monnieri L. cultivated in Iraq. Baghdad Science Journal. 2018. Vol. 15, no. 2, pp.123-129.
https://search.emarefa.net/detail/BIM-837417
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 127-128
Record ID
BIM-837417