Estimation of genetic variations in different taxa in Brassicaceae by RAPD and ISSR analysis
Other Title(s)
تقدير التغايرات الوراثية لمراتب تصنيفية مختلفة من العائلة الصليبية باستخدام تحليل RAPD و ISSR
Author
al-Tamimi, Huda Jasim Muhammad
Source
Bulletin of the Iraq Natural History Museum
Issue
Vol. 15, Issue 1 (30 Jun. 2018), pp.1-13, 13 p.
Publisher
University of Baghdad Iraq Natural History Research Center and Museum
Publication Date
2018-06-30
Country of Publication
Iraq
No. of Pages
13
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Topics
Abstract EN
Twelve species from Brassicaceae family were studied using two different molecular techniques: RAPD and ISSR; both of these techniques were used to detect some molecular markers associated with the genotype identification.
RAPD results, from using five random primers, revealed 241 amplified fragments, 62 of them were polymorphic (26 %).
ISSR results showed that out of seven primers, three (ISSR3, UBC807, UBC811) could not amplify the genomic DNA; other primers revealed 183 amplified fragments, 36 of them were polymorphic (20 %).
The Similarity evidence and dendrogram for the genetic distances of the incorporation between the two techniques showed that the highest similarity was 0.897 between the varieties red cabbage and red ornamental cabbage, meanwhile the lowest similarity index was between the varieties red radish and Green ornamental cabbage (0.169) ; thus these RAPD and ISSR markers have the possibility for the identification of species or varieties and the description of genetic variation within the varieties.
Furthermore, it could be concluded that the Brassicaceae taxa have a suitable amount of genetic variance and a wide range in the genetic principle of the studied genotypes which can be used for output improvement.
American Psychological Association (APA)
al-Tamimi, Huda Jasim Muhammad. 2018. Estimation of genetic variations in different taxa in Brassicaceae by RAPD and ISSR analysis. Bulletin of the Iraq Natural History Museum،Vol. 15, no. 1, pp.1-13.
https://search.emarefa.net/detail/BIM-838984
Modern Language Association (MLA)
al-Tamimi, Huda Jasim Muhammad. Estimation of genetic variations in different taxa in Brassicaceae by RAPD and ISSR analysis. Bulletin of the Iraq Natural History Museum Vol. 15, no. 1 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-838984
American Medical Association (AMA)
al-Tamimi, Huda Jasim Muhammad. Estimation of genetic variations in different taxa in Brassicaceae by RAPD and ISSR analysis. Bulletin of the Iraq Natural History Museum. 2018. Vol. 15, no. 1, pp.1-13.
https://search.emarefa.net/detail/BIM-838984
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 10-12
Record ID
BIM-838984