Determination of vasicine alkaloid efficacy as inhibitor to the activity of protease produced by a clinical isolate of Pseudomonas aeruginosa
Other Title(s)
تحديد فعالية قلويد الفازيسين كمثبط لنشاط البروتيز الذي تنتجه عزلة سريرية من الزوائف الزنجارية
Joint Authors
Jasim, Hibah Abd al-Rahman
Ali, Rua Husayn
Majid, Majid Rashid
Source
Issue
Vol. 59, Issue 3A (30 Sep. 2018), pp.1237-1246, 10 p.
Publisher
University of Baghdad College of Science
Publication Date
2018-09-30
Country of Publication
Iraq
No. of Pages
10
Main Subjects
Abstract EN
In the present study, the effect of vasicine alkaloid separated from Adhatoda vasica as an inhibitor agent on the activity of proteases enzyme isolated from Pseudomonas aeruginosa was investigated.
forty isolates of Pseudomonas aeruginosa were collected from local hospital in Baghdad and then their ability for producing proteases was screened using quantification and semi- quantitative methods.
Pseudomonas aeruginosa P1 was selected as the highest protease producer, which next identified as P.
aeruginosa.
It was found that the optimum culture conditions for protease production in submerged culture was in the tryptic - soya broth medium at 37° C with pH 8 for 48 hours.
In addition, the study involved extraction and partial purification of vacisine alkaloid from crude leaves of Adhatoda vasica by separation and precipitation that was 0.6% of vasicine The results revealed a significant effect of the extracted vasicine on the activity of protease enzyme as its activity was decreased from 15.5U / ml to 1.2 U/ml when treated with vasicine at a concentration of 0.8ml with an inhibitory effect of 92.2%.
American Psychological Association (APA)
Ali, Rua Husayn& Majid, Majid Rashid& Jasim, Hibah Abd al-Rahman. 2018. Determination of vasicine alkaloid efficacy as inhibitor to the activity of protease produced by a clinical isolate of Pseudomonas aeruginosa. Iraqi Journal of Science،Vol. 59, no. 3A, pp.1237-1246.
https://search.emarefa.net/detail/BIM-840285
Modern Language Association (MLA)
Ali, Rua Husayn…[et al.]. Determination of vasicine alkaloid efficacy as inhibitor to the activity of protease produced by a clinical isolate of Pseudomonas aeruginosa. Iraqi Journal of Science Vol. 59, no. 3A (2018), pp.1237-1246.
https://search.emarefa.net/detail/BIM-840285
American Medical Association (AMA)
Ali, Rua Husayn& Majid, Majid Rashid& Jasim, Hibah Abd al-Rahman. Determination of vasicine alkaloid efficacy as inhibitor to the activity of protease produced by a clinical isolate of Pseudomonas aeruginosa. Iraqi Journal of Science. 2018. Vol. 59, no. 3A, pp.1237-1246.
https://search.emarefa.net/detail/BIM-840285
Data Type
Journal Articles
Language
English
Notes
Text in English ; abstracts in English and Arabic.
Record ID
BIM-840285