دراسة 15 نظاما من أنظمة التكرارات الترادفية القصيرة STRs لدى عينة من الشعب السوري

Abstract EN

Background& Objective: The aim of this study was to know the Allele frequencies for the 15 STR loci in the sample in Aleppo in Syria.

Materials & Methods: Allele frequencies for the 15 STR loci included in the PowerPlex16 PCR Amplification Kit panel from (Promega, Madison, USA) according to manufacturer’s instructions (D3S1358, TH01, D21S11, D18S51, PentaE, D5S818, D13S317, D7S820, D16S539, CSF1PO, PenyaD, vWA, D8S1179, T, POX , FGA) and several statistical parameters were estimated from a sample of 127 unrelated individuals representing all ethnic groups in Aleppo in Syria .

DNA was extracted from blood samples using QIAmp1 DNA Mini Kit (QIAGEN, Hagen, Germany) according to manufacturer’s instructions and the PCR products were typed using an ABI PRISM 310 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA)).

The results were analyzed using GeneMapper ID v3.0 software (Applied Biosystems).

Several forensic and population parameters such as heterozygosity, polymorphic information content (PIC), power of discrimination (PD), power of exclusion (PE) and the paternity index (PI)) were calculated using Microsoft Excel-.

A p-value of the 15 loci were estimated by using the statistical Microsoft Windows 2007 to verify whether the genotype distribution conformed to HardyWeinberg equilibrium predictions.

Results : We compared our data with other data sets from published studies of individuals from Turkey, Morocco and Iraq, our data indicate that standardized multilocus STR panels may be a useful forensic tool which can be applied for identification purposes also in the Syria population.

Conclusion: Our Data may be a useful forensic tool which can be applied for identification purposes also in the Syria population.