Identification and quantification of chimerism in bone marrow transplants leukemic patients
Other Title(s)
تحديد و تقدير كمية الكايميرا لمرضى نقل نخاع العظم المصابين بابيضاض الدم الحاد
Joint Authors
al-Zubaydi, Muhammad Mahdi
al-Samirrai, Halah Khalid Ibrahim
Mahmud, Hanan Khalil
Salih, Khalifah M.
Badr, Ala Hazim
al-Mayyah, Qasim Sharhan
Sabbah, Majid Arshid
Source
Iraqi Journal of Cancer and Medical Genetics
Issue
Vol. 10, Issue 1 (30 Jun. 2017), pp.41-47, 7 p.
Publisher
al-Mustansyriah University Iraqi Centre for Cancer and Medical Genetics Research
Publication Date
2017-06-30
Country of Publication
Iraq
No. of Pages
7
Main Subjects
Abstract EN
Quantification of chimerism after bone marrow transplantation is essential for evaluation the successful of engraftment after allogenic stem cells transplantation.
The aim of this work is to use STR typing test for identification and quantification the chimerism in bone marrow transplant patients.
Two patients subjected to bone marrow transplantation were analyzed.
DNA extracted from patients (recipient) blood, cheek swabs and donor cheek swabs.
Quantifiler Real time PCR kit was used for DNA quantification.
Powerplex21 kit was used for amplification STR loci.
STR profiling was performed by Genetic analyzer.
DNA extraction and quantification were successfully performed with suitable quantity and purity.
STR loci fully amplified and analyzed.
In both cases recipient and Donor shared alleles with no DNA mixtures.
Few loci showed Type 3 chimerism.
In conclusion the results showed that the test successfully identify used for identification and quantification of chimerism in recipient patients.
List of abbreviation: DNA= Deoxyribonucleic acid, STR- short tandem repeat, SCT= stem cells transplantation.
American Psychological Association (APA)
Sabbah, Majid Arshid& al-Samirrai, Halah Khalid Ibrahim& al-Zubaydi, Muhammad Mahdi& Mahmud, Hanan Khalil& Salih, Khalifah M.& al-Mayyah, Qasim Sharhan…[et al.]. 2017. Identification and quantification of chimerism in bone marrow transplants leukemic patients. Iraqi Journal of Cancer and Medical Genetics،Vol. 10, no. 1, pp.41-47.
https://search.emarefa.net/detail/BIM-844627
Modern Language Association (MLA)
Sabbah, Majid Arshid…[et al.]. Identification and quantification of chimerism in bone marrow transplants leukemic patients. Iraqi Journal of Cancer and Medical Genetics Vol. 10, no. 1 (2017), pp.41-47.
https://search.emarefa.net/detail/BIM-844627
American Medical Association (AMA)
Sabbah, Majid Arshid& al-Samirrai, Halah Khalid Ibrahim& al-Zubaydi, Muhammad Mahdi& Mahmud, Hanan Khalil& Salih, Khalifah M.& al-Mayyah, Qasim Sharhan…[et al.]. Identification and quantification of chimerism in bone marrow transplants leukemic patients. Iraqi Journal of Cancer and Medical Genetics. 2017. Vol. 10, no. 1, pp.41-47.
https://search.emarefa.net/detail/BIM-844627
Data Type
Journal Articles
Language
English
Notes
Record ID
BIM-844627