Effect of low-level laser irradiation versus room temperature on cryopreserved sperm motility and DNA integrity as modes of thawing
Other Title(s)
تأثير استخدام الليزر كطريقة لإذابة النطف البشرية المجمدة مقابل الإذابة بدرجة حرارة الغرفة على سلامة الأحماض النووية و الحركة
Joint Authors
al-Marayati, Shadha Sadiq
Said, Ghassan Thabit
al-Ahmad, Hazim Ismail Abd al-Bari
Source
Journal of Biotechnology Research Center
Issue
Vol. 11, Issue 2 (31 Dec. 2017), pp.30-36, 7 p.
Publisher
al-Nahrain University Biotechnology Research Center
Publication Date
2017-12-31
Country of Publication
Iraq
No. of Pages
7
Main Subjects
Abstract EN
During cryopreservation, cells and tissue undergo dramatic transformation in chemical and physical characteristics.
Thawing process is also an important step since the cryoinjury is not limited to the freezing process but may also occur during the thawing process.
Laser by its photo-stimulation effect can improve the resistance to cooled storage of some human sperm and this will lead to improvement in the quality of frozen semen and in the potential of sperm fertilization.
The objective of this study is to use laser as a method of thawing and its effect on human sperm motility and DNA integrity versus the thawing through room temperature.
This prospective study carried on 70 cryopreserved semen samples.
Each sample was prepared by centrifugation procedure, and divided into 2 parts, freezed and thawed by laser irradiation till melting for one part and by room temperature for other.
The DNA integrity and sperm motility were assessed before vitrification and after the two methods of thawing.
The results of cryopreserved semen samples showed that laser irradiation thawing has significantly increased sperm motility as well as a significant decreased DNA fragmentation (P< 0.05) versus room temperature thawing.
Conclusion(s): Laser irradiation thawing of post freezing sperm improves post thaw motility and DNA integrity.
American Psychological Association (APA)
al-Marayati, Shadha Sadiq& Said, Ghassan Thabit& al-Ahmad, Hazim Ismail Abd al-Bari. 2017. Effect of low-level laser irradiation versus room temperature on cryopreserved sperm motility and DNA integrity as modes of thawing. Journal of Biotechnology Research Center،Vol. 11, no. 2, pp.30-36.
https://search.emarefa.net/detail/BIM-846996
Modern Language Association (MLA)
al-Marayati, Shadha Sadiq…[et al.]. Effect of low-level laser irradiation versus room temperature on cryopreserved sperm motility and DNA integrity as modes of thawing. Journal of Biotechnology Research Center Vol. 11, no. 2 (Dec. 2017), pp.30-36.
https://search.emarefa.net/detail/BIM-846996
American Medical Association (AMA)
al-Marayati, Shadha Sadiq& Said, Ghassan Thabit& al-Ahmad, Hazim Ismail Abd al-Bari. Effect of low-level laser irradiation versus room temperature on cryopreserved sperm motility and DNA integrity as modes of thawing. Journal of Biotechnology Research Center. 2017. Vol. 11, no. 2, pp.30-36.
https://search.emarefa.net/detail/BIM-846996
Data Type
Journal Articles
Language
English
Notes
Record ID
BIM-846996