In vitro multiplication protocol for sustainable propagation of Harmal (Peganum harmala L.) : a distinguished medicinal wild plant
Other Title(s)
برتوكول تطبيقي للإكثار الدقيق المستدام لنبات الحرمل : نبات بري طبي
Joint Authors
Yunus, Layla Sasi
Zatimah, Ahmad
Hawamidah, Fayha M.
Shibli, Rida Ahmad
al-Qudah, Tamara Said
Tahtamouni, Riham Walid
Source
Jordan Journal of Agricultural Sciences
Issue
Vol. 13, Issue 1 (31 Mar. 2017), pp.25-34, 10 p.
Publisher
University of Jordan Deanship of Academic Research (DAR)
Publication Date
2017-03-31
Country of Publication
Jordan
No. of Pages
10
Main Subjects
Abstract EN
Harmal (Peganum harmala) is a wild plant with a distinguished medicinal potential due to the presence of high levels of phytoalkaloids such as harmaline, harmine and harmalol.
Recently, this plant was reported to posses to antimicrobial, antifungal, anti-inflammatory, anti cancerous hypothermic and hallucinogenic activities.
Meanwhile, short span of seed viability, uncontrolled extensive collection and drought are the main constraints of a sustainable propagation and supply of this important herb.
In this study in vitro propagation of Peganum harmala L.
was successfully achieved via in vitro propagation techniques.
Different types of cytokinin (BA, kinetin, 2ip, or TDZ) at different concentrations (0.0, 0.5, 1.0, 1.5 or 2.0 mg/L) were experimented in terms of their potential promoting new microshoots.
The effects of different types of auxins (IBA, NAA or IAA) on rooting of Harmal microshoots were studied at different levels (0.0, 0.5, 1.0, 1.5 or 2.0 mg/L).
All explants developed new microshoots, and the maximum number of shoots (10.2) and length (8.5 cm) were obtained on medium containing (1.5 mg/L) BA.
Meanwhile, no rooting was observed in the explants grown either in the control or media supplemented with (0.5, 1.0 or 2.0 mg/L) IBA, while maximum root number (8.0) and root length (3.6 cm) were obtained on MS media supplemented with 1.0 mg/L NAA.
Moreover, rooted plantlets were
American Psychological Association (APA)
Zatimah, Ahmad& Shibli, Rida Ahmad& Hawamidah, Fayha M.& Yunus, Layla Sasi& Tahtamouni, Riham Walid& al-Qudah, Tamara Said. 2017. In vitro multiplication protocol for sustainable propagation of Harmal (Peganum harmala L.) : a distinguished medicinal wild plant. Jordan Journal of Agricultural Sciences،Vol. 13, no. 1, pp.25-34.
https://search.emarefa.net/detail/BIM-882172
Modern Language Association (MLA)
Zatimah, Ahmad…[et al.]. In vitro multiplication protocol for sustainable propagation of Harmal (Peganum harmala L.) : a distinguished medicinal wild plant. Jordan Journal of Agricultural Sciences Vol. 13, no. 1 (2017), pp.25-34.
https://search.emarefa.net/detail/BIM-882172
American Medical Association (AMA)
Zatimah, Ahmad& Shibli, Rida Ahmad& Hawamidah, Fayha M.& Yunus, Layla Sasi& Tahtamouni, Riham Walid& al-Qudah, Tamara Said. In vitro multiplication protocol for sustainable propagation of Harmal (Peganum harmala L.) : a distinguished medicinal wild plant. Jordan Journal of Agricultural Sciences. 2017. Vol. 13, no. 1, pp.25-34.
https://search.emarefa.net/detail/BIM-882172
Data Type
Journal Articles
Language
English
Record ID
BIM-882172