The genetic relationship for Klebsiella pneumoniae isolated from human urinary tract and beef
Other Title(s)
العلاقة الوراثية لجرثومة الكلبيسيلا الرئوية و المعزولة من الجهاز البولي في الإنسان و الأبقار
Joint Authors
Klaif, Siba Falah
al-Ubaydi, Janan Nazim Sadiq
Nasir, Hasan Hajim
Source
Iraqi Journal of Veterinary Sciences
Issue
Vol. 33, Issue 1 (30 Jun. 2019), pp.75-80, 6 p.
Publisher
University of Mosul College of Veterinary Medicine
Publication Date
2019-06-30
Country of Publication
Iraq
No. of Pages
6
Main Subjects
Abstract EN
The present study aimed to describe the genetic relationships of zoonotic characterization of Klebsiella pneumoniae isolated from Human urinary tract and beef.
The study includes (50) urine samples from human and (50) beef samples.
The isolation and identification of Klebsiella pneumonia were done by using enrichment culture method and Vitek 2, then confirmed by PCR technique based on 16S ribosomal RNA gene which designed in this study using NCBI-GenBank (LT599801.1) and DNA sequencing was done on some positive isolates.
The results show that Klebsiella pneumoniae was isolated from beef at 38 (76%) and from human at 32 (64%) by vitek2.
The PCR technique was show highly sensitive and specific confirmative detection of Klebsiella Pneumonia isolates at Clarify DNA sequencing of a partial sequence of 16S ribosomal RNA gene was shown homology sequence identity highly with NCBI-Blast Klebsiella pneumoniae isolates.
The phylogenetic analysis was show clear genetic similarity at (0.5 genetic change) between human and beef in Klebsiella pneumoniae isolates.
The gene sequence deposited into NCBI-GenBank accession numbers (MF314450.1, MF314451.1, MF314452.1, MF314453.1).
In conclusion, the study presents the first report in Iraq of genetic relationship among K.
pneumoniae isolates from beef and humans.
Therefore, it is essential to define the role of animals as an important source for the distribution of pathogen related to public health.
American Psychological Association (APA)
Klaif, Siba Falah& Nasir, Hasan Hajim& al-Ubaydi, Janan Nazim Sadiq. 2019. The genetic relationship for Klebsiella pneumoniae isolated from human urinary tract and beef. Iraqi Journal of Veterinary Sciences،Vol. 33, no. 1, pp.75-80.
https://search.emarefa.net/detail/BIM-895297
Modern Language Association (MLA)
Nasir, Hasan Hajim…[et al.]. The genetic relationship for Klebsiella pneumoniae isolated from human urinary tract and beef. Iraqi Journal of Veterinary Sciences Vol. 33, no. 1 (2019), pp.75-80.
https://search.emarefa.net/detail/BIM-895297
American Medical Association (AMA)
Klaif, Siba Falah& Nasir, Hasan Hajim& al-Ubaydi, Janan Nazim Sadiq. The genetic relationship for Klebsiella pneumoniae isolated from human urinary tract and beef. Iraqi Journal of Veterinary Sciences. 2019. Vol. 33, no. 1, pp.75-80.
https://search.emarefa.net/detail/BIM-895297
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 79-80
Record ID
BIM-895297