The interference of Newcastle, avian influenza and infectious bursal disease vaccines with the efficiency of IB vaccine in broiler chicks by using quantitative RT-PCR test
Other Title(s)
تداخل لقاح مرض النيوكاسل و إنفلونزا الطيور و التهاب جراب فابريشيا مع كفاءة لقاح التهاب القصبات المعدي لفروج اللحم باستخدام اختبار Quantative RT-PCR
Joint Authors
al-Musawi, Isam Muhammad Jabir Zabibah
Rabi, Raid Husayn Salih
Source
Iraqi Journal of Veterinary Sciences
Issue
Vol. 33, Issue 1 (30 Jun. 2019), pp.105-109, 5 p.
Publisher
University of Mosul College of Veterinary Medicine
Publication Date
2019-06-30
Country of Publication
Iraq
No. of Pages
5
Main Subjects
Topics
Abstract EN
This study was conducted to investigate the effects the interference of ND, AI, IBD vaccines on efficacy of the IBV vaccination program (H120 and Ma5).
Three hundred broiler chicks (Ross308) from Al-Haddi hatchery / Babel province were divided randomly into three equal groups, each group contain 100 chicks and treated as follows: the 1st group; IB vaccine (H120) was given at one day old by intranasal and ocular drop methods, IB vaccine Ma5 strain vaccine was given at age 14 days by spray method.
The 2nd group at one day old IB H120 + ND LaSota alive vaccines strain was given by intranasal and ocular drop method, also given mixed killed vaccine contain ND with AI by S/C injection.
At 10 days old alive ND vaccine LaSota strain gives by drinking water.
At 12 in day old attenuated IBD vaccine gives by drinking water.
At day old IB Ma5 strain vaccine was given using spray method.
The 3ed group: control group without vaccine.
Blood samples were collected from jugular vein at 1, 7, 14, 21, 28, 35 and 40 day old to determine the antibody titer against IBV by ELISA test and samples were taken from trachea and kidney for Real- time quantitative -PCR (viral load) at 21 and 35 days old.
All groups were challenged with local virulent IBV isolate (IBV CH Babylon F2 2013) 100×106.5 ELD50/ml at 28 days old by ocular and intranasal drop method.
Morbidity and mortality were recorded in all the challenged birds.
The result show that the significant differences between all groups in different parameters at P<0.05.
American Psychological Association (APA)
Rabi, Raid Husayn Salih& al-Musawi, Isam Muhammad Jabir Zabibah. 2019. The interference of Newcastle, avian influenza and infectious bursal disease vaccines with the efficiency of IB vaccine in broiler chicks by using quantitative RT-PCR test. Iraqi Journal of Veterinary Sciences،Vol. 33, no. 1, pp.105-109.
https://search.emarefa.net/detail/BIM-895471
Modern Language Association (MLA)
Rabi, Raid Husayn Salih& al-Musawi, Isam Muhammad Jabir Zabibah. The interference of Newcastle, avian influenza and infectious bursal disease vaccines with the efficiency of IB vaccine in broiler chicks by using quantitative RT-PCR test. Iraqi Journal of Veterinary Sciences Vol. 33, no. 1 (2019), pp.105-109.
https://search.emarefa.net/detail/BIM-895471
American Medical Association (AMA)
Rabi, Raid Husayn Salih& al-Musawi, Isam Muhammad Jabir Zabibah. The interference of Newcastle, avian influenza and infectious bursal disease vaccines with the efficiency of IB vaccine in broiler chicks by using quantitative RT-PCR test. Iraqi Journal of Veterinary Sciences. 2019. Vol. 33, no. 1, pp.105-109.
https://search.emarefa.net/detail/BIM-895471
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 108-109
Record ID
BIM-895471