The effect of growth condition on SAP10 gene expression in genotype A candida albicans
Other Title(s)
تأثير ظروف النمو على التعبير الجيني لجين (SAP10) في النمط الجيني A في لمبيضات البيض
Joint Authors
Rahim, Inas Qasim
Samakah, Haydar Mahmud Ali
Source
Kufa Journal for Veterinary Medical Sciences
Issue
Vol. 10, Issue 1 (30 Jun. 2019), pp.71-78, 8 p.
Publisher
University of Kufa Faculty of Veterinary Medicine
Publication Date
2019-06-30
Country of Publication
Iraq
No. of Pages
8
Main Subjects
Abstract EN
There are a limited data regarding to the role of the growth conditions on pathogenicity process of Candida albicans and if the host play a role in augmenting the virulence of this microorganism, this study focusing on the effect of environmental growth conditions of genotype A Candida albicans on SAP10 gene expression as one of the Secreted Aspartyl Proteinase super family genes that play a main role in Candida albicans pathogenicity process.
Ten pathogenic strains of Candida albicans obtained from clinical cases were included in this study, genotyped according to 25s rDNA and grown on two different condition using RPMI1640 medium at for mimic host condition (under controlled condition in vitro) and also grown on Sabauroud Dextrose agar at 25 as in vitro condition, total RNA were extracted from each condition and evaluated using Pfaffle's equation.
The results of this study exhibit that all tested isolates classified under genotype (A) Candida albicans with 450pb PCR product size of 25s rDNA, while SAP 10 gene expression data indicate that no significant expression pattern related to the different growth conditions and the expressions are related to the tested strains and no relation between Candida albicans growth conditions, strain genotype and SAP10 gene expression is approved in this study
American Psychological Association (APA)
Rahim, Inas Qasim& Samakah, Haydar Mahmud Ali. 2019. The effect of growth condition on SAP10 gene expression in genotype A candida albicans. Kufa Journal for Veterinary Medical Sciences،Vol. 10, no. 1, pp.71-78.
https://search.emarefa.net/detail/BIM-895935
Modern Language Association (MLA)
Rahim, Inas Qasim& Samakah, Haydar Mahmud Ali. The effect of growth condition on SAP10 gene expression in genotype A candida albicans. Kufa Journal for Veterinary Medical Sciences Vol. 10, no. 1 (2019), pp.71-78.
https://search.emarefa.net/detail/BIM-895935
American Medical Association (AMA)
Rahim, Inas Qasim& Samakah, Haydar Mahmud Ali. The effect of growth condition on SAP10 gene expression in genotype A candida albicans. Kufa Journal for Veterinary Medical Sciences. 2019. Vol. 10, no. 1, pp.71-78.
https://search.emarefa.net/detail/BIM-895935
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 76-78
Record ID
BIM-895935