Berberine chloride and hyperthermia promote Osterix expression and suppress cell cycle genes in osteosarcoma cells

Abstract EN

Objectives: The aim of the current study was to investigate the effects of berberine chloride and various heat conditions on the gene expression of Osterix, RUNX-2, RANKL, CDK2, CDK4, IL-6 and IL-11.

Methods: Six groups of cells were treated with hyperthermia for 1 h: Two groups at mild, two at moderate and two at severe hyperthermia (39, 43 and 45°C respectively).

Berberine chloride (80 µg/mL) was selected for treating one group of mild, moderate and severe hyperthermia (combination).

All treated groups were recovered at 37°C for 24 h.

Another exposure for hyperthermia (1 h) and recovery for 3 h at 37°C were applied.

Results: The expression of Osterix was highly upregulated in all groups except in the severe hyperthermia and mild hyperthermia with berberine groups.

Only the mild hyperthermia without berberine induced a slight increase in the expression of RUNX2, whereas severe hyperthermia alone suppressed the levels on a significant manner.

Berberine alone was more effective in significantly up-regulating RANKL expression.

On the other hand, CDK2 mRNA was downregulated in all groups.

CDK4 showed a similar regulation in the mild hyperthermia group with control, but the expression was downregulated in the other groups, especially in severe hyperthermia the expression was significantly downregulated (p<0.5).

IL-6 was upregulated highly and significantly in the group of berberine and all groups of combinations, whereas mild and moderate hyperthermia stimulated significant expression of IL-11 mRNA.