تحفيز البروتين الكلي لثلاث طحالب خضر معزولة محليا

Abstract EN

The current study is an attempt to highlight the possibility of stimulating the amount of total protein of three green algae (Asterococcus spp, Scenedesmus dimorphus, Chlorococcum humicola) under the influence of Nitrogen removal (N2- 0), adding of Sodium chloride (NaCl), mixture of vitamins (B1, B6, B12) and change the current speed (as a mixing factor) of 400 and 850L/h.

Algae were Isolated locally from the Iraqi environment and selected culture medium (Chu-13) for culturing and development with controlled conditions (25 ± 2 ° C, intensity of illumination 3000 Lux and 8:16 light: darkness system and pH 7-7.5.Total protein was determined depending on the methods of Lowry modified by Lopes et al.

( 2010) and then the optical density was measured at a wavelength 665 nm and a standard curve depending on the protein (Bovin serum albumin) concentrations (0-10) ml was draw.

The results showed varying effects on total protein between algae either in control (Chu-13 medium) or when the transaction with the variables of removing nitrogen and adding NaCl, mixture of vitamins B, current speed change, Results were as follows: Algae Asterococcus sp, S.dimorphus and C.

humicola recoded a total protein values (109.6, 78.8 and 185.9) mg/g-1 and percentage (19.5,2 0.17 and 35.3.%) in control medium, Chu-13, respectively, while recorded (111.2, 41.5 and 170.7) mg/g-1 and (27.0, 11.7, 31.7%) in Chu13-N0, and (119.4, 112.3 and 158.6) mg/g-1 and (28.O, 26.7 and 29.3%) in the center Chu13 + NaCl and (225.3, 146.7, 216.2) mg / g -1 and (51.1, 28.9, 40.3%) in Chu13 + (B1, B6, B12) and (294.7, 46.5, 214.3) mg/g-1 and (40.7, 9.1 and 36.9%) at 400 and (171.6, 127, 145.2) mg/g-1, and (31.8, 29.2, 28.4%) at 850 l/h current speed respectively.