Ability of pitavastatin to enhance doxorubicin efficacy Against MCF7 breast cancer cells

Other Title(s)

قدرة عقار البيتافستاتين على تحسين الأثر العلاجي لعقار الدوكسوروبوسين على خلايا سرطان الثدي

Dissertant

Murtaja, Khalid Muhammad Dib

Thesis advisor

al-Bashiti, Tariq Abd al-Qadir
al-Uwayni, Sahib Husayn

University

Islamic University

Faculty

Faculty of Science

Department

Department of Biological Science

University Country

Palestine (Gaza Strip)

Degree

Master

Degree Date

2019

English Abstract

Background: Breast cancer is the most common malignancy in women worldwide. While doxorubicin is part of the standard therapy for metastatic breast cancer, it has limited success. Pitavastatin, has been shown to enhance the anti-cancer activity of certain therapeutics. Objective: This study has been conducted to explore the anti-cancer activity of the combined treatment of doxorubicin and pitavastatin in MCF7 breast cancer cells. Methods: Cell proliferation assay has been employed to evaluate the general toxicity of these compounds in the MCF7 cells cancer cells. The anti-cancer effects of these compounds against cancer cells was assessed using trypan blue assay. To understand the mechanism of action of the doxorubicin and pitavastatin treatment, western blotting analysis was performed. Results: Cell proliferation and viability assays demonstrated that combined doxorubicin and pitavastatin treatment resulted in synergistic cytotoxicity and cell death. Western blotting analysis showed that pitavastatin treatment resulted in increased levels of p53 and the cell cycle regulator p21 in both doxorubicin treated and untreated cells. Furthermore, we demonstrated that apoptosis induced by the combined treatment occurs through the extrinsic pathway as evident from the activation of caspase 8 and the reduction of BCL-2 level. Conclusion: This study provides novel evidence to suggest that combined treatment of doxorubicin and pitavastatin may be effectively combined to treat breast cancer with the potential to minimize the side effects associated with high doses of doxorubicin

Main Topic

Biology

No. of Pages

67

Table of Contents

Table of contents.

Abstract

Abstract in Arabic.

Chapter One : Introduction.

Chapter Two : Literature review.

Chapter Three : Materials and methods.

Chapter Four : Research methodology.

Chapter Five : Discussion.

Chapter Six : Conclusions and recommendations.

References.

American Psychological Association (APA)

Murtaja, Khalid Muhammad Dib. (2019). Ability of pitavastatin to enhance doxorubicin efficacy Against MCF7 breast cancer cells. (Master's theses Theses and Dissertations Master). Islamic University, Palestine (Gaza Strip)
https://search.emarefa.net/detail/BIM-903062

Modern Language Association (MLA)

Murtaja, Khalid Muhammad Dib. Ability of pitavastatin to enhance doxorubicin efficacy Against MCF7 breast cancer cells. (Master's theses Theses and Dissertations Master). Islamic University. (2019).
https://search.emarefa.net/detail/BIM-903062

American Medical Association (AMA)

Murtaja, Khalid Muhammad Dib. (2019). Ability of pitavastatin to enhance doxorubicin efficacy Against MCF7 breast cancer cells. (Master's theses Theses and Dissertations Master). Islamic University, Palestine (Gaza Strip)
https://search.emarefa.net/detail/BIM-903062

Language

English

Data Type

Arab Theses

Record ID

BIM-903062