Effects of a specific nutrient combination on ESBL resistance
Joint Authors
Matar, Ghassan M.
Ayyub, Muhammad Sadiq
Harakeh, Steve
al-Masudi, Sad
Kissoyan, Kohar Berge
Fadl Allah, Sukaynah
Tanelian, Arax
Source
Saudi Journal of Biological Sciences
Issue
Vol. 26, Issue 7 (30 Nov. 2019), pp.1576-1580, 5 p.
Publisher
Publication Date
2019-11-30
Country of Publication
Saudi Arabia
No. of Pages
5
Main Subjects
Topics
Abstract EN
Background and aim Extended-spectrum beta-lactamases are the main cause of resistance in Enterobacteriaceae to beta lactam antibiotics.
The aim of this study was to evaluate the antimicrobial effect of EpiQuercican supplement, combined with different antimicrobial agents, on ESBL-producing isolates and determine the underlying molecular mechanism of resistance in these isolates.
Materials and methods Eleven ESBL producing Enterobacteriaceae isolates were collected from Saudi Arabia hospitals between 2016 and 2017 and disk diffusion test was performed in accordance with Clinical and Laboratory Standards Institute (CLSI) guidelines to determine the susceptibility of the isolates to 5 different antibiotics in the presence of EpiQuercican supplement.
Polymerase chain reaction was performed for detection of ESBL genes, and efflux pump inhibitor was used to study the mechanism of resistance in these isolates.
Results The best synergistic effect was obtained when the supplement was combined with carbapenems followed by 4th generation cephalosporins.
Either no effect or antagonistic effect was seen with most of the isolates when the supplement was added to the 3rd generation of cephalosporins.
Among the tested genes responsible for ESBL production in this study, our results indicated the predominance of TEM genes (73%) followed by CTX-M genes (9%).
As for the mechanism of resistance in ESBL isolates, 4 isolates showed to use efflux pumps as their main mechanism of resistance.
Conclusion The EpiQuercican supplement showed some promising results, yet its antibacterial mechanism of action needs to be elucidated further.
American Psychological Association (APA)
Harakeh, Steve& Ayyub, Muhammad Sadiq& al-Masudi, Sad& Kissoyan, Kohar Berge& Fadl Allah, Sukaynah& Tanelian, Arax…[et al.]. 2019. Effects of a specific nutrient combination on ESBL resistance. Saudi Journal of Biological Sciences،Vol. 26, no. 7, pp.1576-1580.
https://search.emarefa.net/detail/BIM-910177
Modern Language Association (MLA)
Harakeh, Steve…[et al.]. Effects of a specific nutrient combination on ESBL resistance. Saudi Journal of Biological Sciences Vol. 26, no. 7 (Nov. 2019), pp.1576-1580.
https://search.emarefa.net/detail/BIM-910177
American Medical Association (AMA)
Harakeh, Steve& Ayyub, Muhammad Sadiq& al-Masudi, Sad& Kissoyan, Kohar Berge& Fadl Allah, Sukaynah& Tanelian, Arax…[et al.]. Effects of a specific nutrient combination on ESBL resistance. Saudi Journal of Biological Sciences. 2019. Vol. 26, no. 7, pp.1576-1580.
https://search.emarefa.net/detail/BIM-910177
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 1580
Record ID
BIM-910177