Application of high-fat cell model in steady-state regulation of vascular function

Joint Authors

Song, Qinghong
Zhang, Yan

Source

Saudi Journal of Biological Sciences

Issue

Vol. 26, Issue 8 (31 Dec. 2019), pp.2132-2135, 4 p.

Publisher

Saudi Biological Society

Publication Date

2019-12-31

Country of Publication

Saudi Arabia

No. of Pages

4

Main Topic

Biology
Diseases

Topics

Abstract EN

In order to effectively apply the high-fat cell model to the regulation of vascular homeostasis and the repair of vascular endothelial cell injury, and to provide a new theoretical basis for the treatment of vascular homeostasis imbalance in the future, in this study, the mouse thoracic aorta tissue is extracted by using mouse endothelial cells. Western blotting and immunofluorescence resonance energy transfer (Immuno-FRET) are then used to verify the distribution and physical coupling properties of TRPV4 and Nox2 in cells. Finally, mouse mesenteric endothelial cells are isolated and cultured to induce FFA highfat cell model. The results show that the nucleic acid expression levels of TRPV4 and Nox2 in RNA are significantly different from those of TRPV4 and Nox2 in protein. The relative values of TRPV4 and Nox2 in the control group are relatively low (0.8 ± 0.11). However, the relative values of TRPV4 and Nox2 are higher in the FFA high-fat cell model induced by the experimental group, and the values are (1.7 ± 0.8). Obviously, the relative values of TRPV4 and Nox2 in the experimental group are higher than those in the control group. The expression of reactive oxygen species (ROS) in vascular endothelial cells of control group is (1.0 ± 0.16), and that in FFA group is (2.5 ± 0.46). The expression of ROS in FFA cell model with HC067047A inhibitor is (1.5 ± 0.38). In the FFA cell model with apo inhibitor, ROS expression is (1.2 ± 0.23). Thus, in the FFA high-fat cell model induced successfully, the physical coupling of TRPV4 and Nox2 increases in primary endothelial cells, and the increase of physical coupling of TRPV4 and Nox2 results in the increase of ROS expression, which also means the imbalance of ROS homeostasis in vascular endothelial cells and the change of vascular endothelial cell permeability. The expression levels of TRPV4 and Nox2 are used as indicators of whether the vascular function is stable or unbalanced, thus providing a new theoretical basis for the treatment of cardiovascular diseases.

American Psychological Association (APA)

Song, Qinghong& Zhang, Yan. 2019. Application of high-fat cell model in steady-state regulation of vascular function. Saudi Journal of Biological Sciences،Vol. 26, no. 8, pp.2132-2135.
https://search.emarefa.net/detail/BIM-910636

Modern Language Association (MLA)

Song, Qinghong& Zhang, Yan. Application of high-fat cell model in steady-state regulation of vascular function. Saudi Journal of Biological Sciences Vol. 26, no. 8 (Dec. 2019), pp.2132-2135.
https://search.emarefa.net/detail/BIM-910636

American Medical Association (AMA)

Song, Qinghong& Zhang, Yan. Application of high-fat cell model in steady-state regulation of vascular function. Saudi Journal of Biological Sciences. 2019. Vol. 26, no. 8, pp.2132-2135.
https://search.emarefa.net/detail/BIM-910636

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references : p. 2135

Record ID

BIM-910636