Design, preparation and evaluation of Glipizide solid lipid nanoparticles for improving its oral bioavailability

Other Title(s)

تصميم و صياغة و تقييم الجزيئات الدهنية الصلبة في حجم النانومتر لعقار الجليبيزايد لتحسين إتاحته الحيوية عن طريق الفم

Dissertant

Mukhtar, Iman Jamal

Thesis advisor

al-Karad, Jamal al-Yusuf

University

Isra University

Faculty

Faculty of Pharmacy

University Country

Jordan

Degree

Master

Degree Date

2019

English Abstract

The main aim of the present thesis is to develop Glipizide solid lipid nanoparticles (GlipSLNs) for improving its bioavailability, therapeutic efficacy and increasing duration of action.

Eight different formulae of Glip.

-SLNs were produced by HSH and sonication technique.

Solubility study was performed on different lipids including Beeswax, Compritol® 888 ATO, Precirol® ATO 5 and stearic acid to identify appropriate lipids for formulation of Glip.-SLNs.

Particle size, PDI, zeta potential, %DC, %EE, %DL, % PY and in-vitro release parameters (t50 and t90) were taken as responses to detect the optimized formula.

The results indicated solubility of Glip.

in Compritol® 888 ATO and Precirol® ATO 5 which have low Hydrophilic-lipophilic balance (HLB).

A valid HPLC method for analysis of Glip- SLNs showed the retention time of Glip.

was 4.1 min.

and the method was linear over a range of 1-20 µg/ml.

Formula (SLN7) which composed of precirol as lipid base, span 60 as lipid surfactant, lutrol F 127 as aqueous surfactant and methocel E5 (1%) as viscosity increasing agent was the optimized formula.

Particle size, PDI, ZP, % DC, % EE, % DL, % PY, t50 and t90 for optimized formula were 217 nm, 0.307, -13.80 mV, 99.29 %, 93.45%, 60.32%, 95.82%, 17.68 hrs.

and 31.75 hrs.

respectively.

On comparing oral bioavailability of Glip- SLNs (SLN7) with marketed product (Minidiab® 5 mg tablet) as tested by the rate of change in blood glucose level (E) in wistar rats after single oral dose, the bioavailability of (SLN7) has 2.50 folds increase than marketed product.

Results of Liquid Chromatography Mass Spectrometry (LC-MS) for analysis of SN7 and marketed product in plasma of wistar rats showed percentage relative bioavailability (PRBA) of Glip.

- SLNs (SLN 7) in comparison to marketed product (Minidiab® 5 mg tablet) was 413.00 %.

Main Topic

Pharmacology

Topics

No. of Pages

102

Table of Contents

Table of contents.

Abstract.

Abstract in Arabic.

Chapter One : Background of study.

Chapter Two : Literature review.

Chapter Three : Methodology.

Chapter Four : Results and discussion.

Chapter Five : Conclusion

References.

American Psychological Association (APA)

Mukhtar, Iman Jamal. (2019). Design, preparation and evaluation of Glipizide solid lipid nanoparticles for improving its oral bioavailability. (Master's theses Theses and Dissertations Master). Isra University, Jordan
https://search.emarefa.net/detail/BIM-913508

Modern Language Association (MLA)

Mukhtar, Iman Jamal. Design, preparation and evaluation of Glipizide solid lipid nanoparticles for improving its oral bioavailability. (Master's theses Theses and Dissertations Master). Isra University. (2019).
https://search.emarefa.net/detail/BIM-913508

American Medical Association (AMA)

Mukhtar, Iman Jamal. (2019). Design, preparation and evaluation of Glipizide solid lipid nanoparticles for improving its oral bioavailability. (Master's theses Theses and Dissertations Master). Isra University, Jordan
https://search.emarefa.net/detail/BIM-913508

Language

English

Data Type

Arab Theses

Record ID

BIM-913508