Molecular identification of e. coli virulence factors genes, bfp and elt, in feces of diarrheic chickens
Other Title(s)
التشخيص الجزيئي لجينات عوامل الضراوة، BFB و elt ، للايشريكيا القولونية في براز الدجاج المصاب بالإسهال
Source
Journal of al-Qadisiyah for Pure Science
Issue
Vol. 23, Issue 2 (30 Jun. 2018), pp.17-29, 13 p.
Publisher
al-Qadisiyah University College of Science
Publication Date
2018-06-30
Country of Publication
Iraq
No. of Pages
13
Main Subjects
Abstract EN
The study was aimed to demonstrate the existence of major virulence factors bfp and elt genes for enteropathogenic e.
coli (epec) and enterotoxogenic e.
coli (etec), respectively, in feces of diarrheic chicken by using pcr technique.
for this purpose, 50 diarrheic chickens were subjected for fecal samples collection.
in first, the samples have been cultured on macconkey and eosin methylene blue agars, and the isolates were tested by some specific biochemical tests to verify of e.
coli samples, which revealed on 20 positive samples.
the twenty positive samples were submitted, only, for pcr technique to confirm the existence of elt and bfp genes.
with genes detection, the overall results of the present study were showed that 11/50 (22 %) and 7/50 (14 %) were positives for epec-bfp and etec-elt genes, respectively.
also, the cross-infections results with bfp and elt genes had been identified that 5/20 (25%) were positives with both genes, 2/20 (10 %) were positives with elt gene, only, and 6/20 (30%) were positives with bfp gene, only.
statistically, the significant differences were reported between the prevalence rates of bfp and elt genes, at a level of p0.05.
American Psychological Association (APA)
al-Ubaydi, Janan Nazim Sadiq& al-Muhammad, Siba Abbud Ali& al-Zaydi, Rana Mashal Salim. 2018. Molecular identification of e. coli virulence factors genes, bfp and elt, in feces of diarrheic chickens. Journal of al-Qadisiyah for Pure Science،Vol. 23, no. 2, pp.17-29.
https://search.emarefa.net/detail/BIM-914139
Modern Language Association (MLA)
al-Muhammad, Siba Abbud Ali…[et al.]. Molecular identification of e. coli virulence factors genes, bfp and elt, in feces of diarrheic chickens. Journal of al-Qadisiyah for Pure Science Vol. 23, no. 2 (2018), pp.17-29.
https://search.emarefa.net/detail/BIM-914139
American Medical Association (AMA)
al-Ubaydi, Janan Nazim Sadiq& al-Muhammad, Siba Abbud Ali& al-Zaydi, Rana Mashal Salim. Molecular identification of e. coli virulence factors genes, bfp and elt, in feces of diarrheic chickens. Journal of al-Qadisiyah for Pure Science. 2018. Vol. 23, no. 2, pp.17-29.
https://search.emarefa.net/detail/BIM-914139
Data Type
Journal Articles
Language
English
Record ID
BIM-914139