Molecular identification and diversity analysis of dental bacteria in diabetic and non-diabetic females from Saudi Arabia

Joint Authors

Faden, Asma A.
al-Ubaydah, Muhammad I.
al-Nakhli, Ala K. M.
al-Utaybi, Sahar
al-Id, Bushra
Ekhzaimy, Aishah
Arif, Ibrahim A.
Khan, Hasib Ahmad

Source

Saudi Journal of Biological Sciences

Issue

Vol. 27, Issue 1 (31 Jan. 2020), pp.358-362, 5 p.

Publisher

Saudi Biological Society

Publication Date

2020-01-31

Country of Publication

Saudi Arabia

No. of Pages

5

Main Topic

Biology

Topics

Abstract EN

Periodontal disease is a chronic infectious disease, which is characterized by the damaged dental hard tissue by lactic acid generated by microorganisms after the fermentation of carbohydrates rich diet. The risk of periodontal disease is known to be higher in diabetic patients. We compared the diversity of five commonly occurring dental bacteria including Porphyromonas gingivalis, Tannerella forsythia, Capnocytophaga ochracea, Prevotella intermedia, and Aggregatibacter actinomycetemcomitans in 14 type-2 diabetic patients and equal numbers of healthy controls. The subgingival samples were collected using sterile paper points. We used 16S rRNA sequence specific primers for PCR-based identification of dental bacteria. Our results showed that A. actinomycetemcomitans was completely absent in control subjects but present in 43% of diabetic patients. C. ochracea was highly prevalent in diabetic patients (100%) as compared to controls (28.5%). The frequency of other three bacterial species was also higher in diabetic patients than control subjects. These findings indicate that dental bacteria are highly prevalent in subgingival pockets of diabetic patients. Therefore, proper monitoring of diabetic patients for dental care is important to prevent bacterial growth and its sequela in risky individuals. Further case-control studies using larger sample size would help in validating the association between oral diseases and diabetes.

American Psychological Association (APA)

al-Ubaydah, Muhammad I.& al-Nakhli, Ala K. M.& Arif, Ibrahim A.& Faden, Asma A.& al-Utaybi, Sahar& Khan, Hasib Ahmad…[et al.]. 2020. Molecular identification and diversity analysis of dental bacteria in diabetic and non-diabetic females from Saudi Arabia. Saudi Journal of Biological Sciences،Vol. 27, no. 1, pp.358-362.
https://search.emarefa.net/detail/BIM-920673

Modern Language Association (MLA)

Arif, Ibrahim A.…[et al.]. Molecular identification and diversity analysis of dental bacteria in diabetic and non-diabetic females from Saudi Arabia. Saudi Journal of Biological Sciences Vol. 27, no. 1 (Jan. 2020), pp.358-362.
https://search.emarefa.net/detail/BIM-920673

American Medical Association (AMA)

al-Ubaydah, Muhammad I.& al-Nakhli, Ala K. M.& Arif, Ibrahim A.& Faden, Asma A.& al-Utaybi, Sahar& Khan, Hasib Ahmad…[et al.]. Molecular identification and diversity analysis of dental bacteria in diabetic and non-diabetic females from Saudi Arabia. Saudi Journal of Biological Sciences. 2020. Vol. 27, no. 1, pp.358-362.
https://search.emarefa.net/detail/BIM-920673

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references : p. 362

Record ID

BIM-920673