Molecular and immunological methods to confirm Toxiginicity (Microcystin production) of Westiellopsis Prolifica isolated from Tigris river-Iraq
Other Title(s)
طرق تقدير جزيئية و مناعية لتأكيد القابلية السمية (إنتاج المايكروسستين) من طحلب المعزول من مياه نهر دجلة-العراق Westiellopsis prolifica
Joint Authors
Abd, Ibrahim Jabir
al-Sudani, Ghusun Ali Abd al-Hasan
Mushib, Layth Ibrahim
Source
Issue
Vol. 16, Issue 4 (sup) (31 Dec. 2019), pp.978-985, 8 p.
Publisher
University of Baghdad College of Science for Women
Publication Date
2019-12-31
Country of Publication
Iraq
No. of Pages
8
Main Subjects
Abstract EN
Several toxigenic cyanobacteria produce the cyanotoxin (microcystin).
Being a health and environmental hazard, screening of water sources for the presence of microcystin is increasingly becoming a recommended environmental procedure in many countries of the world.
This study was conducted to assess the ability of freshwater cyanobacterial species Westiellopsis prolifica to produce microcystins in Iraqi freshwaters via using molecular and immunological tools.
The toxigenicity of W.
prolifica was compared via laboratory experiments with other dominant bloom-forming cyanobacteria isolated from the Tigris River: Microcystis aeruginosa, Chroococcus turigidus, Nostoc carneum, and Lyngbya sp.
significant expression of mcyE gene and microcystin production was most evident in W.
prolifica.
Contrary to the prevailing concept that M.
aeruginosa is a main microcystin producer in freshwaters around the world, no significant microcystin production was observed with this species throughout the time points studied in our laboratory methods.
As for C.
turigidus, N.
carneum and Lyngbya sp., neither mcyE expression nor microcystin production was significant.
Data from mcyE expression by RT-qPCR were generally in agreement with those obtained from microcystin quantification by ELISA.
Interestingly, W.
prolifica, which showed clear microcystin-producing ability in this study and which was not reported before in the literature to produce microcystin, can be added as a new microcystin producer to the list of toxigenic cyanobacteria.
American Psychological Association (APA)
Abd, Ibrahim Jabir& al-Sudani, Ghusun Ali Abd al-Hasan& Mushib, Layth Ibrahim. 2019. Molecular and immunological methods to confirm Toxiginicity (Microcystin production) of Westiellopsis Prolifica isolated from Tigris river-Iraq. Baghdad Science Journal،Vol. 16, no. 4 (sup), pp.978-985.
https://search.emarefa.net/detail/BIM-935354
Modern Language Association (MLA)
Abd, Ibrahim Jabir…[et al.]. Molecular and immunological methods to confirm Toxiginicity (Microcystin production) of Westiellopsis Prolifica isolated from Tigris river-Iraq. Baghdad Science Journal Vol. 16, no. 4 (Supplement) (2019), pp.978-985.
https://search.emarefa.net/detail/BIM-935354
American Medical Association (AMA)
Abd, Ibrahim Jabir& al-Sudani, Ghusun Ali Abd al-Hasan& Mushib, Layth Ibrahim. Molecular and immunological methods to confirm Toxiginicity (Microcystin production) of Westiellopsis Prolifica isolated from Tigris river-Iraq. Baghdad Science Journal. 2019. Vol. 16, no. 4 (sup), pp.978-985.
https://search.emarefa.net/detail/BIM-935354
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 984-985
Record ID
BIM-935354