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Detection of methicillin-resistant staphylococcus aureus in broiler and broilers farm workers in Duhok, Iraq by using conventional and PCR techniques
Other Title(s)
تحديد المكورات العنقودية الذهبية المقاومة للمثيسيلين في الدواجن و عمال حقول الدواجن في دهوك-العراق باستخدام الطرق التقليدية و تفاعل البلمرة المتسلسل
Joint Authors
al-Assafi, Mahdi Salih
Haddu, Hushyar Abd al-Jabbar
Abd al-Rahman, Ibtisam Salih
Source
Iraqi Journal of Veterinary Sciences
Issue
Vol. 34, Issue 1 (30 Jun. 2020), pp.15-22, 8 p.
Publisher
University of Mosul College of Veterinary Medicine
Publication Date
2020-06-30
Country of Publication
Iraq
No. of Pages
8
Main Subjects
Topics
Abstract EN
Livestock-associated methicillin-resistant Staphylococcus aureus (MRSA) has become a global public health concern.
The purpose of this study was to investigate the prevalence rates of MRSA infection amongst broiler chickens and broilers farm workers.
The total samples used in this study were 306.
Cloacal swab samples from 231 broilers and nasal swab samples from 75 broilers farm workers were collected from five farms in Duhok city, Iraq.
Isolation and identification of MRSA isolates were carried out and the antibiotic susceptibility were screened.
Molecular characterization of all isolates was performed by using polymerase chain reaction (PCR) technique to detect the mecA gene.
S.
aureus was detected among 84% (63/75) of the farms workers samples and among 84.8% (196/231) of the broiler's samples.
The S.
aureus isolated from farm workers and broilers appeared resistant to oxacillin 28.6% (18/63), and 32.1% (63/196), respectively.
MRSA colonization in farm workers and broilers was 24% (18/75) and 27.3% (63/231) respectively.
The S.
aureus isolates showed the most resistant to chloramphenicol and the least resistant to vancomycin.
The results of the PCR assays revealed that 85.7% (12/14) of S.
aureus isolates from farm workers and 44.4% (16/36) of S.
aureus isolates from broilers were positive for the mecA gene.
The direct handling of broilers by farm workers plays the important role for transport the MRSA isolates from broilers to broilers farm workers.
American Psychological Association (APA)
al-Assafi, Mahdi Salih& Haddu, Hushyar Abd al-Jabbar& Abd al-Rahman, Ibtisam Salih. 2020. Detection of methicillin-resistant staphylococcus aureus in broiler and broilers farm workers in Duhok, Iraq by using conventional and PCR techniques. Iraqi Journal of Veterinary Sciences،Vol. 34, no. 1, pp.15-22.
https://search.emarefa.net/detail/BIM-948781
Modern Language Association (MLA)
al-Assafi, Mahdi Salih…[et al.]. Detection of methicillin-resistant staphylococcus aureus in broiler and broilers farm workers in Duhok, Iraq by using conventional and PCR techniques. Iraqi Journal of Veterinary Sciences Vol. 34, no. 1 (2020), pp.15-22.
https://search.emarefa.net/detail/BIM-948781
American Medical Association (AMA)
al-Assafi, Mahdi Salih& Haddu, Hushyar Abd al-Jabbar& Abd al-Rahman, Ibtisam Salih. Detection of methicillin-resistant staphylococcus aureus in broiler and broilers farm workers in Duhok, Iraq by using conventional and PCR techniques. Iraqi Journal of Veterinary Sciences. 2020. Vol. 34, no. 1, pp.15-22.
https://search.emarefa.net/detail/BIM-948781
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 20-22
Record ID
BIM-948781