Detection of methicillin-resistant staphylococcus aureus in broiler and broilers farm workers in Duhok, Iraq by using conventional and PCR techniques

Other Title(s)

تحديد المكورات العنقودية الذهبية المقاومة للمثيسيلين في الدواجن و عمال حقول الدواجن في دهوك-العراق باستخدام الطرق التقليدية و تفاعل البلمرة المتسلسل

Joint Authors

al-Assafi, Mahdi Salih
Haddu, Hushyar Abd al-Jabbar
Abd al-Rahman, Ibtisam Salih

Source

Iraqi Journal of Veterinary Sciences

Issue

Vol. 34, Issue 1 (30 Jun. 2020), pp.15-22, 8 p.

Publisher

University of Mosul College of Veterinary Medicine

Publication Date

2020-06-30

Country of Publication

Iraq

No. of Pages

8

Main Topic

Veterinary Medicine

Abstract EN

Livestock-associated methicillin-resistant Staphylococcus aureus (MRSA) has become a global public health concern.

The purpose of this study was to investigate the prevalence rates of MRSA infection amongst broiler chickens and broilers farm workers.

The total samples used in this study were 306.

Cloacal swab samples from 231 broilers and nasal swab samples from 75 broilers farm workers were collected from five farms in Duhok city, Iraq.

Isolation and identification of MRSA isolates were carried out and the antibiotic susceptibility were screened.

Molecular characterization of all isolates was performed by using polymerase chain reaction (PCR) technique to detect the mecA gene.

S.

aureus was detected among 84% (63/75) of the farms workers samples and among 84.8% (196/231) of the broiler's samples.

The S.

aureus isolated from farm workers and broilers appeared resistant to oxacillin 28.6% (18/63), and 32.1% (63/196), respectively.

MRSA colonization in farm workers and broilers was 24% (18/75) and 27.3% (63/231) respectively.

The S.

aureus isolates showed the most resistant to chloramphenicol and the least resistant to vancomycin.

The results of the PCR assays revealed that 85.7% (12/14) of S.

aureus isolates from farm workers and 44.4% (16/36) of S.

aureus isolates from broilers were positive for the mecA gene.

The direct handling of broilers by farm workers plays the important role for transport the MRSA isolates from broilers to broilers farm workers.

American Psychological Association (APA)

al-Assafi, Mahdi Salih& Haddu, Hushyar Abd al-Jabbar& Abd al-Rahman, Ibtisam Salih. 2020. Detection of methicillin-resistant staphylococcus aureus in broiler and broilers farm workers in Duhok, Iraq by using conventional and PCR techniques. Iraqi Journal of Veterinary Sciences،Vol. 34, no. 1, pp.15-22.
https://search.emarefa.net/detail/BIM-948781

Modern Language Association (MLA)

al-Assafi, Mahdi Salih…[et al.]. Detection of methicillin-resistant staphylococcus aureus in broiler and broilers farm workers in Duhok, Iraq by using conventional and PCR techniques. Iraqi Journal of Veterinary Sciences Vol. 34, no. 1 (2020), pp.15-22.
https://search.emarefa.net/detail/BIM-948781

American Medical Association (AMA)

al-Assafi, Mahdi Salih& Haddu, Hushyar Abd al-Jabbar& Abd al-Rahman, Ibtisam Salih. Detection of methicillin-resistant staphylococcus aureus in broiler and broilers farm workers in Duhok, Iraq by using conventional and PCR techniques. Iraqi Journal of Veterinary Sciences. 2020. Vol. 34, no. 1, pp.15-22.
https://search.emarefa.net/detail/BIM-948781

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references : p. 20-22

Record ID

BIM-948781