Detection of the extended spectrum β-lactamase produced by Escherichia coli from dairy cows by using the Vitek-2 method in Tulungagung regency, Indonesia

Other Title(s)

الكشف عن إنزيم بتا-اكتام ممتد الطيف المنتج من الايشيريكا القولونية من أبقار حلوب بوساطة طريقة ال Vitek-2 في تولونجاجونج، اندنوسيا

Joint Authors

Putra, A. R.
Effendi, Mustafa Hilmi
Koesdarto, S.
Suwarno, S.
Tyasningsih, W.
Estoepangestie, A. T.

Source

Iraqi Journal of Veterinary Sciences

Issue

Vol. 34, Issue 1 (30 Jun. 2020), pp.203-207, 5 p.

Publisher

University of Mosul College of Veterinary Medicine

Publication Date

2020-06-30

Country of Publication

Iraq

No. of Pages

5

Main Topic

Veterinary Medicine

Abstract EN

The use of antibiotics in inappropriate on food producing animals can lead to resistance many of the pathogenic bacteria to the various types of antibiotics, one of which is the Escherichia coli (E. coli) which produces extended spectrum β-lactamase (ESBL). Antibiotic resistance in animals and humans has become a global problem that needs attention and immediate management by using specific antibiotics that used for therapeutic the infected animals. The aim of this study was to isolate and detect E. coli producing ESBL. All E. coli from the surface of dairy cow rectal swabs in Sendang District, Tulungagung Regency, Indonesia using the Vitek-2 method. The number of rectal swab samples used in the present study was 50. The results of this study showed that all the samples were suspected of being E. coli, based on the morphological growth of colonies on the EMBA media. The isolates were identified by using the biochemical tests. All the samples were positive. In this study the double disc synergy test (DDST) method was using to confirm the ESBL. The antibiotics were used amoxicylyn-clavulanate, ceftazidime and cefotaxime for DDST. In additional ESBL confirmation test was used the Vitek-2 method. The presence of ESBL producing by E. coli isolated from rectal dairy swabs in tulungagung was 6% (3/50)

American Psychological Association (APA)

Putra, A. R.& Effendi, Mustafa Hilmi& Koesdarto, S.& Suwarno, S.& Tyasningsih, W.& Estoepangestie, A. T.. 2020. Detection of the extended spectrum β-lactamase produced by Escherichia coli from dairy cows by using the Vitek-2 method in Tulungagung regency, Indonesia. Iraqi Journal of Veterinary Sciences،Vol. 34, no. 1, pp.203-207.
https://search.emarefa.net/detail/BIM-948807

Modern Language Association (MLA)

Putra, A. R.…[et al.]. Detection of the extended spectrum β-lactamase produced by Escherichia coli from dairy cows by using the Vitek-2 method in Tulungagung regency, Indonesia. Iraqi Journal of Veterinary Sciences Vol. 34, no. 1 (2020), pp.203-207.
https://search.emarefa.net/detail/BIM-948807

American Medical Association (AMA)

Putra, A. R.& Effendi, Mustafa Hilmi& Koesdarto, S.& Suwarno, S.& Tyasningsih, W.& Estoepangestie, A. T.. Detection of the extended spectrum β-lactamase produced by Escherichia coli from dairy cows by using the Vitek-2 method in Tulungagung regency, Indonesia. Iraqi Journal of Veterinary Sciences. 2020. Vol. 34, no. 1, pp.203-207.
https://search.emarefa.net/detail/BIM-948807

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references : p. 206-207

Record ID

BIM-948807