Detection of mitochondrial cytochrome C oxidase subunit I (COI)‎ gene as a molecular marker for identification local Rhopalosiphum aphid (Homoptera : Aphididae)‎

Abstract EN

Aphid species are usually identified by microscopic examination of morphological features.

This process requires significant expertise and is difficult in immature aphid stages that lack many diagnostic morphological features in addition to the characterization of polymorphism to this group so resolution is required the molecular techniques for aphid diagnosis.

Since there were no molecular data or specific primers used to identify the local species.

Thus, the current study aimed to detect of mitochondrial cytochrome C oxidase subunit I gene as a molecular marker.

Lep primer was applied to local Rhopalosiphum aphids as the most common primer for aphid samples identification which were previously characterized by morphological features.

Only 5% of samples was not be detected by this primer.

We conclude the importance for sequencing and analysis of DNA sequences to design a specific primer for local Rhopalosiphum aphids that could improve the misidentification problems that are intrinsic with morphological features.