Genetic characterization of Egyptian field isolates of infectious bursal disease virus
Other Title(s)
التوصيف الجزيئي لمرض الاتهاب الكيس الفيبريشي المعدي
Joint Authors
Salim, Abd Allah Abd al-Zahir
Hamid, Dalya M.
Shibl, Iman..
Abidin.Hanan, M. F
Source
Suez Canal Veterinary Medicine Journal
Issue
Vol. 22, Issue 1 (30 Jun. 2017), pp.79-91, 13 p.
Publisher
Suez Canal University Faculty of Veterinary Medicine
Publication Date
2017-06-30
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Detection and characterization of field infectious bursal disease virus (IBDV) isolates circulating among different broiler breeds representing three Egyptian governorates with a history of vaccination against IBDV, was done during 2013- 2015.
Reverse transcriptase polymerase chain reaction (RT-PCR) was used to amplify and produce clear band of 620 Base pair (bp) product within the hypervariable region of the IBDV viral protein (VP2) gene.
The IBDV was prevalent in thirty out one hundred flocks (30%).
Subsequently, chosen PCR products were subjected to further analysis by sequencing and phylogenetic studies.
Amino acid substitution in VP2 region used to establish a clear framework on the epidemiology of isolated IBDV .
Genetic analysis showed that all examined IBDV isolates characterized as vvIBDV had amino acids residues(222A, 242I, 256I, 294I, 299S) which showed to be unique for all vvIBDV strains and clustered phylogenetically with the previously Egyptian IBDVs ( 99.3-100% ) identity.
While, vaccinal IBDV strains (BURSA-VAC and UNIVAX) were clustered in another group with (91.1–91.9%), (91.9–92.6%) identity respectively which might leads to vaccination failure and reemergence of disease.
Consequently, we recorded wide range of genetic similarities (94.8-95.6) with the recombinant vaccines (VP2 of Faragher 52/70) seed virus, which showed superior protective efficacy against recent Egyptian vvIBDV isolates.
IBDV is still a threat against poultry industry.
It is important to keep track on appearance and evolution of antigenically different IBDV circulating strains followed by regular updating of the vaccination strategy.
Key words: vvIBDV, phenotypic characterization, vaccinati
American Psychological Association (APA)
Shibl, Iman..& Abidin.Hanan, M. F; Abidin.Hanan, M. F& Hamid, Dalya M.& Salim, Abd Allah Abd al-Zahir. 2017. Genetic characterization of Egyptian field isolates of infectious bursal disease virus. Suez Canal Veterinary Medicine Journal،Vol. 22, no. 1, pp.79-91.
https://search.emarefa.net/detail/BIM-959822
Modern Language Association (MLA)
Abidin.Hanan, M. F…[et al.]. Genetic characterization of Egyptian field isolates of infectious bursal disease virus. Suez Canal Veterinary Medicine Journal Vol. 22, no. 1 (2017), pp.79-91.
https://search.emarefa.net/detail/BIM-959822
American Medical Association (AMA)
Shibl, Iman..& Abidin.Hanan, M. F; Abidin.Hanan, M. F& Hamid, Dalya M.& Salim, Abd Allah Abd al-Zahir. Genetic characterization of Egyptian field isolates of infectious bursal disease virus. Suez Canal Veterinary Medicine Journal. 2017. Vol. 22, no. 1, pp.79-91.
https://search.emarefa.net/detail/BIM-959822
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 88-91
Record ID
BIM-959822