توصيف العزلات السورية لفيروسي البقع الحلقية الميتة للخوخ-للبرقوق و جدري الخوخ- البرقوق

Abstract EN

To obtain preliminary evidence concerning strain diversity, 15 PNRSV isolates from three different Prunus hosts (Almond, Peach and Plum) were chosen for studying their biological, serological and molecular characteristics.

Usualy, non-specific symptoms for each PNRSV isolate were observed on herbaceous and woody indicator plants.

PNRSV transmission from infected scions to GF305 rootstocks occurred two weeks after grafting and were confirmed by indirect ELISA.

The PPV ELISA-positive apricot sample was grafted on GF305 and symptoms similar to those induced by PPV were obtained.

PNRSV isolates tested by DASI-ELISA using 10 PNRSV-specific MAbs showed that there were some serological differences between Syrian PNRSV isolates, which were identified as four PNRSV serogroups.

DASI-ELISA using 4 serotype-specific MAbs and universal MAb (Control) showed that the PPV isolate tested belonged to the PPV-M strain.

The results of RT-PCR of 9 PNRSV isolates were in a agreement with the serological data.

However, single-strand conformation polymorphism (SSCP) and restriction fragment length polymorphism (RFLP) analysis confirmed substantially a high homology among all the tested PNRSV isolates.