Comparative study of some virulence factors and analysis of phylogenetic tree by 16s rDNA sequencing of Aeromonas hydrophila isolated from clinical and environmental samples
Other Title(s)
دراسة مقارنة في بعض عوامل الضراوة و تحليل الشجرة الجينية التطورية بواسطة تسلسل قواعد 16s rDNA لبكتريا Aeromonas hydrophila المعزولة من العينات السريرية و البيئية
Joint Authors
al-Sudani, Ghusun Ali Abd al-Hasan
al-Attar, Naghm Shakir
Jaddu, Nihad Taha Muhammad
Source
Issue
Vol. 60, Issue 11 (30 Nov. 2019), pp.2390-2398, 9 p.
Publisher
University of Baghdad College of Science
Publication Date
2019-11-30
Country of Publication
Iraq
No. of Pages
9
Main Subjects
Abstract EN
Aeromonas hydrophila is widely distributed throughout the world and causes diseases to animals and human exposed to contaminated environments such as water and soil.
This study aimed to compare between isolates of A.
hydrophila collected from clinical and environmental samples, through investigating the phenotype of some virulence factors in vitro, including hemolysin, protease, lipase, nuclease and biofilm formation ability.
Also, the antimicrobial susceptibility for different antibiotics was determined using disc diffusion method.
For genotypic identification of isolates and phylogenetic tree construction, 16S rDNA target gene was amplified and sequenced.
The phenotypic results showed some differences between the isolates (clinical and environmental).
All isolates were resistance to clindamycin, amoxicillin and erythromycin while susceptible to gentamicin, amikacin and vancomycin.
Sequences of 16S rDNA confirmed the identification of the studied bacteria as A.
hydrophila with 99-100% , and identity and phylogenetic tree by neighbor-joining clearly separated the isolates in a branching pattern which displayed similarity to the GenBank isolates obtained from Asian regions.
The clinical isolates showed less polymorphism than the environmental isolates.
American Psychological Association (APA)
al-Sudani, Ghusun Ali Abd al-Hasan& al-Attar, Naghm Shakir& Jaddu, Nihad Taha Muhammad. 2019. Comparative study of some virulence factors and analysis of phylogenetic tree by 16s rDNA sequencing of Aeromonas hydrophila isolated from clinical and environmental samples. Iraqi Journal of Science،Vol. 60, no. 11, pp.2390-2398.
https://search.emarefa.net/detail/BIM-969426
Modern Language Association (MLA)
al-Sudani, Ghusun Ali Abd al-Hasan…[et al.]. Comparative study of some virulence factors and analysis of phylogenetic tree by 16s rDNA sequencing of Aeromonas hydrophila isolated from clinical and environmental samples. Iraqi Journal of Science Vol. 60, no. 11 (2019), pp.2390-2398.
https://search.emarefa.net/detail/BIM-969426
American Medical Association (AMA)
al-Sudani, Ghusun Ali Abd al-Hasan& al-Attar, Naghm Shakir& Jaddu, Nihad Taha Muhammad. Comparative study of some virulence factors and analysis of phylogenetic tree by 16s rDNA sequencing of Aeromonas hydrophila isolated from clinical and environmental samples. Iraqi Journal of Science. 2019. Vol. 60, no. 11, pp.2390-2398.
https://search.emarefa.net/detail/BIM-969426
Data Type
Journal Articles
Language
English
Notes
Text in English ; abstracts in English and Arabic.
Record ID
BIM-969426