Molecular identification and phylogenetic analysis of lactic acid bacteria isolated from goat raw milk

Other Title(s)

التشخيص الجزيئي و التحليل النسلي لبكتريا حامض اللاكتيك المعزولة من حليب الماعز الخام في العراق

Joint Authors

Said, Zahrah Kazim
Uthman, Rasha Mundhir
Abbas, Basil Abd al-Zahrah

Source

Iraqi Journal of Veterinary Sciences

Issue

Vol. 34, Issue 2 (31 Dec. 2020), pp.259-263, 5 p.

Publisher

University of Mosul College of Veterinary Medicine

Publication Date

2020-12-31

Country of Publication

Iraq

No. of Pages

5

Main Topic

Veterinary Medicine

Topics

Abstract AR

هدفت هذه الدراسة إلى دراسة التباين الوراثي لجراثيم حامض اللاكتيك و المعزولة من حليب الماعز.

حيث تم جمع 100 عينه من الحليب من مختلف أسواق البصرة و تم زرع هذه العينات على الوسط المحفز لنمو هذه الجراثيم.

بينت النتائج عن وجود 64 عزله من هذه الجراثيم و التي أعطت ايجابية لصبغة غرام و سلبية لأنزيم الكاتالايز بنسبة 64%.

لقد تم تشخيص جميع العزلات باستخدام تفاعل تسلسل البلمرة لجين 16 اس الرايبوزي.

و بينت النتائج عزل 9 سلالات تعود الى اللاكتوكوكس و 6 سلالات تعود الى اللاكتوباسيلس و كانت جميع السلالات المختبرة لتتابع القواعد النتروجينية تشابه بنسبة 99% مع تلك المسجلة في بنك الجينات العالمي.

Abstract EN

The aim of this study was to identify the genetic diversity of lactic acid bacteria (LAB) isolated from the local goat's milk.

A total of 100 raw milk samples were collected from the different Basrah local markets.

All the samples were cultured in the De man, Rogosa, and Sharpe (MRS) medium which enhances the growth of lactic acid bacteria.

The result of the study showed that the only 64 lactic acid bacteria isolated gave the Gram-positive and catalase-negative were 64 (64%).

All the suspected isolates were detected and identified by using polymerase chain reaction (PCR) targeting the 16S rRNA gene and DNA sequencing.

The sequencing results showed that 9 strains belong to Lactococcus spp.

and 6 strains belong to Lactobacillus spp.

and all tested isolates had similarity over 99% with those recorded in the GenBank of The National Centre for Biotechnology.

American Psychological Association (APA)

Said, Zahrah Kazim& Abbas, Basil Abd al-Zahrah& Uthman, Rasha Mundhir. 2020. Molecular identification and phylogenetic analysis of lactic acid bacteria isolated from goat raw milk. Iraqi Journal of Veterinary Sciences،Vol. 34, no. 2, pp.259-263.
https://search.emarefa.net/detail/BIM-973290

Modern Language Association (MLA)

Said, Zahrah Kazim…[et al.]. Molecular identification and phylogenetic analysis of lactic acid bacteria isolated from goat raw milk. Iraqi Journal of Veterinary Sciences Vol. 34, no. 2 (2020), pp.259-263.
https://search.emarefa.net/detail/BIM-973290

American Medical Association (AMA)

Said, Zahrah Kazim& Abbas, Basil Abd al-Zahrah& Uthman, Rasha Mundhir. Molecular identification and phylogenetic analysis of lactic acid bacteria isolated from goat raw milk. Iraqi Journal of Veterinary Sciences. 2020. Vol. 34, no. 2, pp.259-263.
https://search.emarefa.net/detail/BIM-973290

Data Type

Journal Articles

Language

English

Notes

ZIncludes bibliographical references : p. 262-263

Record ID

BIM-973290