Molecular identification and phylogenetic analysis of lactic acid bacteria isolated from goat raw milk
Other Title(s)
التشخيص الجزيئي و التحليل النسلي لبكتريا حامض اللاكتيك المعزولة من حليب الماعز الخام في العراق
Joint Authors
Said, Zahrah Kazim
al-Salam, Rasha Mundhir Uthman
Abbas, Basil Abd al-Zahrah
Source
Iraqi Journal of Veterinary Sciences
Issue
Vol. 34, Issue 2 (31 Dec. 2020), pp.259-263, 5 p.
Publisher
University of Mosul College of Veterinary Medicine
Publication Date
2020-12-31
Country of Publication
Iraq
No. of Pages
5
Main Subjects
Topics
Abstract AR
هدفت هذه الدراسة إلى دراسة التباين الوراثي لجراثيم حامض اللاكتيك و المعزولة من حليب الماعز.
حيث تم جمع 100 عينه من الحليب من مختلف أسواق البصرة و تم زرع هذه العينات على الوسط المحفز لنمو هذه الجراثيم.
بينت النتائج عن وجود 64 عزله من هذه الجراثيم و التي أعطت ايجابية لصبغة غرام و سلبية لأنزيم الكاتالايز بنسبة 64%.
لقد تم تشخيص جميع العزلات باستخدام تفاعل تسلسل البلمرة لجين 16 اس الرايبوزي.
و بينت النتائج عزل 9 سلالات تعود الى اللاكتوكوكس و 6 سلالات تعود الى اللاكتوباسيلس و كانت جميع السلالات المختبرة لتتابع القواعد النتروجينية تشابه بنسبة 99% مع تلك المسجلة في بنك الجينات العالمي.
Abstract EN
The aim of this study was to identify the genetic diversity of lactic acid bacteria (LAB) isolated from the local goat's milk.
A total of 100 raw milk samples were collected from the different Basrah local markets.
All the samples were cultured in the De man, Rogosa, and Sharpe (MRS) medium which enhances the growth of lactic acid bacteria.
The result of the study showed that the only 64 lactic acid bacteria isolated gave the Gram-positive and catalase-negative were 64 (64%).
All the suspected isolates were detected and identified by using polymerase chain reaction (PCR) targeting the 16S rRNA gene and DNA sequencing.
The sequencing results showed that 9 strains belong to Lactococcus spp.
and 6 strains belong to Lactobacillus spp.
and all tested isolates had similarity over 99% with those recorded in the GenBank of The National Centre for Biotechnology.
American Psychological Association (APA)
Said, Zahrah Kazim& Abbas, Basil Abd al-Zahrah& al-Salam, Rasha Mundhir Uthman. 2020. Molecular identification and phylogenetic analysis of lactic acid bacteria isolated from goat raw milk. Iraqi Journal of Veterinary Sciences،Vol. 34, no. 2, pp.259-263.
https://search.emarefa.net/detail/BIM-973290
Modern Language Association (MLA)
Said, Zahrah Kazim…[et al.]. Molecular identification and phylogenetic analysis of lactic acid bacteria isolated from goat raw milk. Iraqi Journal of Veterinary Sciences Vol. 34, no. 2 (2020), pp.259-263.
https://search.emarefa.net/detail/BIM-973290
American Medical Association (AMA)
Said, Zahrah Kazim& Abbas, Basil Abd al-Zahrah& al-Salam, Rasha Mundhir Uthman. Molecular identification and phylogenetic analysis of lactic acid bacteria isolated from goat raw milk. Iraqi Journal of Veterinary Sciences. 2020. Vol. 34, no. 2, pp.259-263.
https://search.emarefa.net/detail/BIM-973290
Data Type
Journal Articles
Language
English
Notes
ZIncludes bibliographical references : p. 262-263
Record ID
BIM-973290
