Identification and discrimination of mycobacterium tuberculosis complex with traditional and real-time PCR in different specimens in Iraq

Other Title(s)

تشخيص و تفريق بكتريا التدرن من مختلف النماذج باستخدام الطرق التقليدية و تفاعل البوليميراز المتسلسل اللحظي في العراق

Joint Authors

Mankhi, Ahmad Asmar
Ali, Shayma Mahmud
al-Fahham, Muhammad Abd al-Ikhwah

Source

Journal of the Faculty of Medicine Baghdad

Issue

Vol. 62, Issue 3 (30 Sep. 2020), pp.85-91, 7 p.

Publisher

University of Baghdad Faculty of Medicine

Publication Date

2020-09-30

Country of Publication

Iraq

No. of Pages

7

Main Topic

Diseases
Medicine

Topics

Abstract AR

خلفية البحث: يعتبر مرض التدرن من المشاكل الصحية العالمية و من المسببات الاساسية للمراضة و الوفاة في معظم انحاء العالم.

فهو المسبب التاسعللوفيات في العالم بالرغم من وجود عدة استراتيجيات للعلاج.

هدف الدراسة: تقييم الطرق التقليدية )المسحة المباشرة و الزرع( مقارنة بفحص تفاعل البوليميراز المتسلسل اللحظي لتشخيص التدرن الرئوي و خارجالرئوي.

المرضى و طرق العمل: تم جمع 612 عينة توزعت الى 409 عينة تدرن رئوي و 203 عينة خارج رئوي من المرضى المراجعين للمختبرالمرجعيللتدرن التابع لمركز الامراض الصدرية و التنفسية التخصصي في بغداد خلال الفترة من الاول من اياروحتى الاول من تشرين الاول.2019 استخدمالفحص المباشر للمسحة و الزرع على وسط لوينشتاين-جنسن و كذلك فحص تفاعل البوليميراز المتسلسل اللحظي لتشخيص التدرن.

النتائج: من مجموع ) 612 ( عينة اظهرت نتائج الفحص المجهري المباشر للمسحات المصبوغة بصبغة زيل-نيلسون ثم الزرع بعدها على و سطلوينشتاين- جنسن ان (% 13.4) 82 عينة كانت ايجابية للفحص المباشر في حين 90 (% 14.7) عينة كانت ايجابية للزرع على وسط لوينشتاين- جنسن.

تم استخلاص الجينوم (DNA) من 90 عينة رئوية و خارج رئوية الايجابية الزرع على الوسط الصلب للدراسة الجزيئية باستخدام تفاعل البوليميرازالمتسلسل اللحظي لتشخيص عائدية البكتريا الى جنس.

Mycobacterium tuberculosis complex اظهرت النتائج وجود 71 مريضا(% 78.9) مصاب ب M.

tuberculosis و ثلاث حالات (% 3.3) مصابة ب M.

bovis مع M.

tuberculosis وحالة واحدة (% 1.1) لمريضمصاب بخليط

Abstract EN

Background: Tuberculosis (TB) is a major public health issue and a main cause of global morbidity and mortality.

TB is the world's ninth leading cause of death despite the numerous treatment strategies for managing the disease.

Objective: To assess the traditional method (direct smear examination and culture) against real-time PCR as pulmonary and extrapulmonary tuberculosis laboratory diagnostic techniques.

Cases and methods: Samples were collected from (612) TB cases, (409) of whom were pulmonary tuberculosis (PTB) and (203) were extrapolmonary tuberculosis (EPTB).

The cases were seeking care at the Specialized Chest and Respiratory Disease Center/ National Reference Laboratory for Tuberculosis (NRL) in Baghdad, during the period 1st of May -1st of October 2019.

Direct smear examination, Lowenstein-Jensen culture and Real Time PCR were used to diagnose TB.

Results: Out of 612 samples received, 82(13.4% ) were positive by smear microscopy, while 90(14.7% ) were able to grow on Lowenstein-Jensen (LJ) media.

Ninety DNA extracts from the samples which were positive on LJ media and 25 control specimens, were diagnosed with molecular analysis by using real time PCR to determine the species of Mycobacterium tuberculosis complex.

The results revealed that the 71 samples (78.9% ) were M.

tuberculosis, three specimens (3.3% ) were combined M.

bovis and M.

tuberculosis, and one M.

tuberculosis, M.

bovis, and M.

bovis BCG, while15 (16.7% ) were negative and subsequently excluded from study.

Conclusion: The comparison between molecular diagnostic methods by using Real time PCR with conventional diagnostic methods, provides a new promising technique and is potentially a practical and rapid alternative to the slower traditional pulmonary and EPTB diagnostic culture.

The results show M.

bovis overall contribution on human TB in comparison to M.

tuberculosis is minor among PTB and EPTB cases in the sample studied.

American Psychological Association (APA)

Ali, Shayma Mahmud& al-Fahham, Muhammad Abd al-Ikhwah& Mankhi, Ahmad Asmar. 2020. Identification and discrimination of mycobacterium tuberculosis complex with traditional and real-time PCR in different specimens in Iraq. Journal of the Faculty of Medicine Baghdad،Vol. 62, no. 3, pp.85-91.
https://search.emarefa.net/detail/BIM-978060

Modern Language Association (MLA)

al-Fahham, Muhammad Abd al-Ikhwah…[et al.]. Identification and discrimination of mycobacterium tuberculosis complex with traditional and real-time PCR in different specimens in Iraq. Journal of the Faculty of Medicine Baghdad Vol. 62, no. 3 (2020), pp.85-91.
https://search.emarefa.net/detail/BIM-978060

American Medical Association (AMA)

Ali, Shayma Mahmud& al-Fahham, Muhammad Abd al-Ikhwah& Mankhi, Ahmad Asmar. Identification and discrimination of mycobacterium tuberculosis complex with traditional and real-time PCR in different specimens in Iraq. Journal of the Faculty of Medicine Baghdad. 2020. Vol. 62, no. 3, pp.85-91.
https://search.emarefa.net/detail/BIM-978060

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references : p. 89-91

Record ID

BIM-978060