LPS Increases MUC5AC by TACETGF-αEGFR Pathway in Human Intrahepatic Biliary Epithelial Cell
Joint Authors
Liu, Zipei
Tian, Feng
Feng, Xiaobin
He, Yu
Jiang, Peng
Li, Jianwei
Guo, Fei
Zhao, Xin
Chang, Hong
Wang, Shuguang
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-08-20
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Mucin 5AC (MUC5AC) overproduction plays important roles in stone formation and recurrence of hepatolithiasis.
We aim to investigate the involved mechanism and the potential target to block this process.
Methods.
42 bile duct samples from hepatolithiasis and 15 normal bile duct samples from hemangioma patients were collected for detecting MUC5AC expression by immunohistochemistry.
MUC5AC and phosphoepidermal growth factor receptor (pEGFR) expressions in human intrahepatic biliary epithelial cells (HIBECs) cultured with or without lipopolysaccharide (LPS) were detected by real-time PCR and western blot analysis.
Transforming growth factor-α (TGF-α) secretion in HIBECs was detected by ELISA.
Results.
MUC5AC was overexpressed in bile ducts of hepatolithiasis samples compared with bile ducts from hemangioma samples.
LPS upregulated MUC5AC expression in HIBECs.
LPS promoted EGFR activation, and inhibiting EGFR activation by AG1478 significantly decreased LPS-induced MUC5AC overexpression in HIBECs.
Moreover, LPS increased TGF-α secretion, and inhibiting tumor necrosis factor-α converting enzyme (TACE), which has been implicated in ectodomain cleavage of TGF-α, significantly inhibited LPS-induced EGFR activation and subsequent MUC5AC overexpression in HIBECs.
Conclusion.
Our results suggested that LPS increases MUC5AC expression through the TACE/TGF-α/EGFR pathway in HIBECs.
This new finding might give light to the prevention of stone formation and recurrence of hepatolithiasis.
American Psychological Association (APA)
Liu, Zipei& Tian, Feng& Feng, Xiaobin& He, Yu& Jiang, Peng& Li, Jianwei…[et al.]. 2013. LPS Increases MUC5AC by TACETGF-αEGFR Pathway in Human Intrahepatic Biliary Epithelial Cell. BioMed Research International،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1003585
Modern Language Association (MLA)
Liu, Zipei…[et al.]. LPS Increases MUC5AC by TACETGF-αEGFR Pathway in Human Intrahepatic Biliary Epithelial Cell. BioMed Research International No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1003585
American Medical Association (AMA)
Liu, Zipei& Tian, Feng& Feng, Xiaobin& He, Yu& Jiang, Peng& Li, Jianwei…[et al.]. LPS Increases MUC5AC by TACETGF-αEGFR Pathway in Human Intrahepatic Biliary Epithelial Cell. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1003585
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003585