The Escherichia coli-Derived Thymosin β4 Concatemer Promotes Cell Proliferation and Healing Wound in Mice
Joint Authors
Wang, Xiaolei
Yang, Guihua
Li, Shanshuang
Gao, Meifeng
Zhao, Pangfeng
Zhao, Lingxia
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-19
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Thymosin β4 (Tβ4) is one of the most promising thymosins for future clinical applications, and it is anticipated that commercial demand for Tβ4 will increase.
In order to develop a new approach to produce recombinant Tβ4, a 168 bp DNA (termed Tβ4) was designed based on the Tβ4 protein sequence and used to express a 4 × Tβ4 concatemer (four tandem copies of Tβ4, termed 4 × Tβ4) together with a histidine tag (6 × His) in E.
coli (strain BL21).
SDS-PAGE and western blot analysis were used to confirm that a recombinant 4 × Tβ4 protein of the expected size (30.87 kDa) was produced following the induction of the bacterial cultures with isopropyl β-D-thiogalactoside (IPTG).
The E.
coli-derived 4 × Tβ4 was purified by Ni-NTA resin, and its activities were examined with regard to both stimulating proliferation of the mice spleen cells in vitro and in vivo wound healing.
The results demonstrate that these activities of the E.
coli-derived recombinant 4 × Tβ4 were similar or even better than existing commercially obtained Tβ4.
This production strategy therefore represents a potentially valuable approach for future commercial production of recombinant Tβ4.
American Psychological Association (APA)
Wang, Xiaolei& Yang, Guihua& Li, Shanshuang& Gao, Meifeng& Zhao, Pangfeng& Zhao, Lingxia. 2013. The Escherichia coli-Derived Thymosin β4 Concatemer Promotes Cell Proliferation and Healing Wound in Mice. BioMed Research International،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1003771
Modern Language Association (MLA)
Wang, Xiaolei…[et al.]. The Escherichia coli-Derived Thymosin β4 Concatemer Promotes Cell Proliferation and Healing Wound in Mice. BioMed Research International No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1003771
American Medical Association (AMA)
Wang, Xiaolei& Yang, Guihua& Li, Shanshuang& Gao, Meifeng& Zhao, Pangfeng& Zhao, Lingxia. The Escherichia coli-Derived Thymosin β4 Concatemer Promotes Cell Proliferation and Healing Wound in Mice. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1003771
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003771