Late Adherent Human Bone Marrow Stromal Cells Form Bone and Restore the Hematopoietic Microenvironment In Vivo
Joint Authors
Vianna, Verônica Fernandes
Bonfim, Danielle Cabral
Cavalcanti, Amanda dos Santos
Fernandes, Marco Cury
Kahn, Suzana Assad
Casado, Priscila Ladeira
Lima, Inayá Correa
Murray, Samuel S.
Murray, Elsa J. Brochmann
Duarte, Maria Eugenia Leite
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-04-24
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Bone marrow stromal cells (BMSCs) are a valuable resource for skeletal regenerative medicine because of their osteogenic potential.
In spite of the very general term “stem cell,” this population of cells is far from homogeneous, and different BMSCs clones have greatly different phenotypic properties and, therefore, potentially different therapeutic potential.
Adherence to a culture flask surface is a primary defining characteristic of BMSCs.
We hypothesized that based on the adherence time we could obtain an enriched population of cells with a greater therapeutic potential.
We characterized two populations of bone marrow-derived cells, those that adhered by three days (R-cells) and those that did not adhere by three days but did by six days (L-cells).
Clones derived from L-cells could be induced into adipogenic, chondrogenic, and osteogenic differentiation in vitro.
L-cells appeared to have greater proliferative capacity, as manifested by larger colony diameter and clones with higher CD146 expression.
Only clones from L-cells developed bone marrow stroma in vivo.
We conclude that the use of late adherence of BMSCs is one parameter that can be used to enrich for cells that will constitute a superior final product for cell therapy in orthopedics.
American Psychological Association (APA)
Vianna, Verônica Fernandes& Bonfim, Danielle Cabral& Cavalcanti, Amanda dos Santos& Fernandes, Marco Cury& Kahn, Suzana Assad& Casado, Priscila Ladeira…[et al.]. 2013. Late Adherent Human Bone Marrow Stromal Cells Form Bone and Restore the Hematopoietic Microenvironment In Vivo. BioMed Research International،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1005121
Modern Language Association (MLA)
Vianna, Verônica Fernandes…[et al.]. Late Adherent Human Bone Marrow Stromal Cells Form Bone and Restore the Hematopoietic Microenvironment In Vivo. BioMed Research International No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-1005121
American Medical Association (AMA)
Vianna, Verônica Fernandes& Bonfim, Danielle Cabral& Cavalcanti, Amanda dos Santos& Fernandes, Marco Cury& Kahn, Suzana Assad& Casado, Priscila Ladeira…[et al.]. Late Adherent Human Bone Marrow Stromal Cells Form Bone and Restore the Hematopoietic Microenvironment In Vivo. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1005121
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1005121