Different Peripheral Tissue Injury Induces Differential Phenotypic Changes of Spinal Activated Microglia
Joint Authors
Tan, Yong-Hui
Fu, Kai-Yuan
Light, Alan R.
Li, Kai
Source
Journal of Immunology Research
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-29
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The purpose of this study is to investigate the possible different cellular marker expression associated with spinal cord microglial activation in different pain models.
Immunohistochemistry and western blotting analysis of CD45, CD68, and MHC class I antigen as well as CD11b and Iba-1 in the spinal cord were quantitatively compared among widely used three pain animal models, complete Freund's adjuvant (CFA) injection, formalin injection, and chronic constriction injury (CCI) models.
The results showed that significant upregulated expressions of CD45 and MHC class I antigen in spinal microglia as well as morphological changes with increased staining with CD11b and Iba-1 were seen in CCI and formalin models and not found in CFA-induced inflammatory pain model.
CD68 expression was only detected in CCI model.
Our findings suggested that different peripheral tissue injuries produced differential phenotypic changes associated with spinal microglial activation; peripheral nerve injury might induce spinal microglia to acquire these immunomolecular phenotypic changes.
American Psychological Association (APA)
Li, Kai& Tan, Yong-Hui& Light, Alan R.& Fu, Kai-Yuan. 2013. Different Peripheral Tissue Injury Induces Differential Phenotypic Changes of Spinal Activated Microglia. Journal of Immunology Research،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1006996
Modern Language Association (MLA)
Li, Kai…[et al.]. Different Peripheral Tissue Injury Induces Differential Phenotypic Changes of Spinal Activated Microglia. Journal of Immunology Research No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1006996
American Medical Association (AMA)
Li, Kai& Tan, Yong-Hui& Light, Alan R.& Fu, Kai-Yuan. Different Peripheral Tissue Injury Induces Differential Phenotypic Changes of Spinal Activated Microglia. Journal of Immunology Research. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1006996
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1006996