RhD Specific Antibodies Are Not Detectable in HLA-DRB11501* Mice Challenged with Human RhD Positive Erythrocytes
Joint Authors
Bernardo, Lidice
Denomme, Gregory A.
Shah, Kunjlata
Lazarus, Alan H.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-12-31
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The ability to study the immune response to the RhD antigen in the prevention of hemolytic disease of the fetus and newborn has been hampered by the lack of a mouse model of RhD immunization.
However, the ability of transgenic mice expressing human HLA DRB11501* to respond to immunization with purified RhD has allowed this question to be revisited.
In this work we aimed at inducing anti-RhD antibodies by administering human RhD+ RBCs to mice transgenic for the human HLA DRB11501* as well as to several standard inbred and outbred laboratory strains including C57BL/6, DBA1/J, CFW(SW), CD1(ICR), and NSA(CF-1).
DRB11501* mice were additionally immunized with putative extracellular immunogenic RhD peptides.
DRB11501* mice immunized with RhD+ erythrocytes developed an erythrocyte-reactive antibody response.
Antibodies specific for RhD could not however be detected by flow cytometry.
Despite this, DRB11501* mice were capable of recognizing immunogenic sequences of Rh as injection with Rh peptides induced antibodies reactive with RhD sequences, consistent with the presence of B cell repertoires capable of recognizing RhD.
We conclude that while HLA DRB11501* transgenic mice may have the capability of responding to immunogenic sequences within RhD, an immune response to human RBC expressing RhD is not directly observed.
American Psychological Association (APA)
Bernardo, Lidice& Denomme, Gregory A.& Shah, Kunjlata& Lazarus, Alan H.. 2014. RhD Specific Antibodies Are Not Detectable in HLA-DRB11501* Mice Challenged with Human RhD Positive Erythrocytes. Advances in Hematology،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1015339
Modern Language Association (MLA)
Bernardo, Lidice…[et al.]. RhD Specific Antibodies Are Not Detectable in HLA-DRB11501* Mice Challenged with Human RhD Positive Erythrocytes. Advances in Hematology No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1015339
American Medical Association (AMA)
Bernardo, Lidice& Denomme, Gregory A.& Shah, Kunjlata& Lazarus, Alan H.. RhD Specific Antibodies Are Not Detectable in HLA-DRB11501* Mice Challenged with Human RhD Positive Erythrocytes. Advances in Hematology. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1015339
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1015339