A Longitudinal Low Dose μCT Analysis of Bone Healing in Mice: A Pilot Study
Joint Authors
Di, Lu-Zhao
Couture, Vanessa
Leblanc, Élisabeth
Alinejad, Yasaman
Beaudoin, Jean-François
Lecomte, Roger
Berthod, François
Faucheux, Nathalie
Balg, Frédéric
Grenier, Guillaume
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-11-06
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Low dose microcomputed tomography (μCT) is a recently matured technique that enables the study of longitudinal bone healing and the testing of experimental treatments for bone repair.
This imaging technique has been used for studying craniofacial repair in mice but not in an orthopedic context.
This is mainly due to the size of the defects (approximately 1.0 mm) in long bone, which heal rapidly and may thus negatively impact the assessment of the effectiveness of experimental treatments.
We developed a longitudinal low dose μCT scan analysis method combined with a new image segmentation and extraction software using Hounsfield unit (HU) scores to quantitatively monitor bone healing in small femoral cortical defects in live mice.
We were able to reproducibly quantify bone healing longitudinally over time with three observers.
We used high speed intramedullary reaming to prolong healing in order to circumvent the rapid healing typical of small defects.
Bone healing prolongation combined with μCT imaging to study small bone defects in live mice thus shows potential as a promising tool for future preclinical research on bone healing.
American Psychological Association (APA)
Di, Lu-Zhao& Couture, Vanessa& Leblanc, Élisabeth& Alinejad, Yasaman& Beaudoin, Jean-François& Lecomte, Roger…[et al.]. 2014. A Longitudinal Low Dose μCT Analysis of Bone Healing in Mice: A Pilot Study. Advances in Orthopedics،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1015973
Modern Language Association (MLA)
Di, Lu-Zhao…[et al.]. A Longitudinal Low Dose μCT Analysis of Bone Healing in Mice: A Pilot Study. Advances in Orthopedics No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1015973
American Medical Association (AMA)
Di, Lu-Zhao& Couture, Vanessa& Leblanc, Élisabeth& Alinejad, Yasaman& Beaudoin, Jean-François& Lecomte, Roger…[et al.]. A Longitudinal Low Dose μCT Analysis of Bone Healing in Mice: A Pilot Study. Advances in Orthopedics. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1015973
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1015973