Effect of Nourishing “Yin”-Removing “Fire” Chinese Herbal Mixture on Hypothalamic NKBNK3R Expression in Female Precocious Rats
Joint Authors
Wang, Shiran
Zhu, Liting
Yu, Jian
Tian, Zhanzhuang
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-16
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Aim.
The present study aims to investigate the effects of nourishing “Yin”-removing “Fire” Chinese herb mixture on the hypothalamic NKB/NK3R expression in female precocious model rats.
Materials and Methods.
Female Sprague-Dawley rats were randomly divided into four groups: normal (N), central precocious puberty (CPP) model (M), CPP fed with Chinese herbal mixture (CHM), and CPP fed with normal saline (MS).
Rats on postnatal day 5 were given a single subcutaneous injection of 300 μg to establish CPP model rats.
Rats of CHM and MS groups were continuously administered with nourishing “Yin”-removing “Fire” Chinese herb mixture or saline since postnatal day 15.
The expressions of hypothalamic NKB/NK3R were detected by means of real-time PCR, western blot, and immunofluorescence histochemistry.
Results.
The day of vaginal opening and establishment of two regular estrous cycles were delayed in the CHM group compared with M and MS groups.
The expression of hypothalamic NKB/NK3R mRNA and protein in the arcuate nucleus (ARC) and medial preoptic (MPO) area were decreased significantly in the CHM group compared with the M and MS groups on the day of onset-puberty.
Conclusions.
These results indicate that the NKB/NK3R signaling pathway might be involved in the effect of herbal mixture treatment on CPP.
American Psychological Association (APA)
Wang, Shiran& Zhu, Liting& Yu, Jian& Tian, Zhanzhuang. 2014. Effect of Nourishing “Yin”-Removing “Fire” Chinese Herbal Mixture on Hypothalamic NKBNK3R Expression in Female Precocious Rats. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1018247
Modern Language Association (MLA)
Wang, Shiran…[et al.]. Effect of Nourishing “Yin”-Removing “Fire” Chinese Herbal Mixture on Hypothalamic NKBNK3R Expression in Female Precocious Rats. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1018247
American Medical Association (AMA)
Wang, Shiran& Zhu, Liting& Yu, Jian& Tian, Zhanzhuang. Effect of Nourishing “Yin”-Removing “Fire” Chinese Herbal Mixture on Hypothalamic NKBNK3R Expression in Female Precocious Rats. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1018247
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1018247