Puerarin Attenuates Cerebral Damage by Improving Cerebral Microcirculation in Spontaneously Hypertensive Rats
Joint Authors
Wu, Xu-Dong
Wang, Chen
Zhang, Zhen-Ying
Fu, Yan
Liu, Feng-Ying
Liu, Xiu-Hua
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-13
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Puerariae Lobatae Radix (Gegen in Chinese) is the dried root of Pueraria lobata, a semiwoody, perennial, and leguminous vine native to China.
Puerarin is one of the effective components of isoflavones isolated from the root of Pueraria lobata.
Previous studies showed that extracts derived from the root of Pueraria lobata possessed antihypertensive effect.
Our study is to investigate whether puerarin contributes to prevention of stroke by improving cerebral microcirculation in rats.
Materials and Methods.
Video microscopy and laser Doppler perfusion imaging on the pia mater were used to measure the diameter of microvessel and blood perfusion in 12-week old spontaneously hypertensive rats (SHRs) and age-matched normotensive WKY rats.
Histological alterations were observed by hematoxylin and eosin staining, and microvessel density in cerebral tissue was measured by immunohistochemical analysis with anti-Factor VIII antibody.
Cell proliferation was detected by [3H]-TdR incorporation, and activities of p42/44 mitogen activated protein kinases (p42/44 MAPKs) were detected by western blot analysis in cultured cerebral microvascular endothelial cells (MECs).
Results.
Intravenous injection of puerarin relaxed arterioles and increased the blood flow perfusion in the pia mater in SHRs.
Puerarin treatment for 14 days reduced the blood pressure to a normal level in SHRs ( P < 0.05 ) and increased the arteriole diameter in the pia mater significantly as compared with vehicle treatment.
Arteriole remodeling, edema, and ischemia in cerebral tissue were attenuated in puerarin-treated SHRs.
Microvessel density in cerebral tissue was greater with puerarin than with vehicle treatment.
Puerarin-treated MECs showed greater proliferation and p42/44 MAPKs activities than vehicle treatment.
Conclusions.
Puerarin possesses effects of antihypertension and stroke prevention by improved microcirculation in SHRs, which results from the increase in cerebral blood perfusion both by arteriole relaxation and p42/44 MAPKs-mediated angiogenesis.
American Psychological Association (APA)
Wu, Xu-Dong& Wang, Chen& Zhang, Zhen-Ying& Fu, Yan& Liu, Feng-Ying& Liu, Xiu-Hua. 2014. Puerarin Attenuates Cerebral Damage by Improving Cerebral Microcirculation in Spontaneously Hypertensive Rats. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1018452
Modern Language Association (MLA)
Wu, Xu-Dong…[et al.]. Puerarin Attenuates Cerebral Damage by Improving Cerebral Microcirculation in Spontaneously Hypertensive Rats. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1018452
American Medical Association (AMA)
Wu, Xu-Dong& Wang, Chen& Zhang, Zhen-Ying& Fu, Yan& Liu, Feng-Ying& Liu, Xiu-Hua. Puerarin Attenuates Cerebral Damage by Improving Cerebral Microcirculation in Spontaneously Hypertensive Rats. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1018452
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1018452