In Vitro and In Vivo Characterization of the New Analgesic Combination Beta-Caryophyllene and Docosahexaenoic Acid
Joint Authors
Fiorenzani, Paolo
Lamponi, Stefania
Magnani, Agnese
Ceccarelli, Ilaria
Aloisi, Anna Maria
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-07
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Beta-caryophyllene (BCP) and docosahexaenoic acid (DHA) are components of several plants with documented anti-inflammatory and analgesic effects in animal pain models.
In the present study, in vitro and in vivo tests were carried out to evaluate their effects, alone or in combination, during long-lasting administration in a model of persistent pain.
IR spectra of the two compounds were obtained to determine their chemical stability and then in vitro toxicity was evaluated in fibroblasts and astrocytes.
In the in vivo tests, the analgesic effects of BCP and BCP+DHA were determined in male rats subjected to a model of persistent recurrent pain (three repetitions of the formalin test once a week) to mimic recurrent pain.
Both substances were administered per os in almond oil for 2 weeks.
Gonadal hormones were determined at the end of the tests to evaluate treatment-induced effects on their levels.
BCP changed fibroblast and astrocyte survival in a dose-dependent manner and the effect was counteracted by DHA coadministration.
In the in vivo tests, pain responses were significantly decreased in the BCP and BCP+DHA groups with respect to OIL after 1 and 2 weeks of treatment.
Estradiol and testosterone levels were increased only in the BCP group.
In conclusion, BCP alone or at lower concentration in combination with DHA was efficacious in modulating pain, showing a clear analgesic activity.
American Psychological Association (APA)
Fiorenzani, Paolo& Lamponi, Stefania& Magnani, Agnese& Ceccarelli, Ilaria& Aloisi, Anna Maria. 2014. In Vitro and In Vivo Characterization of the New Analgesic Combination Beta-Caryophyllene and Docosahexaenoic Acid. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1018719
Modern Language Association (MLA)
Fiorenzani, Paolo…[et al.]. In Vitro and In Vivo Characterization of the New Analgesic Combination Beta-Caryophyllene and Docosahexaenoic Acid. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-1018719
American Medical Association (AMA)
Fiorenzani, Paolo& Lamponi, Stefania& Magnani, Agnese& Ceccarelli, Ilaria& Aloisi, Anna Maria. In Vitro and In Vivo Characterization of the New Analgesic Combination Beta-Caryophyllene and Docosahexaenoic Acid. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1018719
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1018719