mRNA-Expression of ERα, ERβ, and PR in Clonal Stem Cell Cultures Obtained from Human Endometrial Biopsies
Joint Authors
Schüring, A. N.
Braun, J.
Wüllner, S.
Kiesel, L.
Gotte, Martin
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-10-18
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Background.
Proliferation and differentiation of the endometrium are regulated by estrogen and progesterone.
The enormous regenerative capacity of the endometrium is thought to be based on the activity of adult stem cells.
However, information on endocrine regulatory mechanisms in human endometrial stem cells is scarce.
In the present study, we investigated the expression of ERα, ERβ, and PR in clonal cultures of human endometrial stem cells derived from transcervical biopsies.
Methods.
Endometrial tissue of 11 patients was obtained by transcervical biopsy.
Stromal cell suspensions were plated at clonal density and incubated for 15 days.
Expression of ERα, ERβ and PR was determined by qPCR prior to and after one cloning round, and normalized to 18 S rRNA expression.
Results.
Expression of ERα and ERβ was downregulated by 64% and 89%, respectively (P=0.002 and P<0.001).
In contrast, PR was not significantly downregulated, due to a more heterogenous expression pattern.
Conclusions.
Culture of human endometrial stroma cells results in a downregulation of ERα and ERβ, while expression of PR remained unchanged in our patient collective.
These results support the hypothesis that stem cells may not be subject to direct stimulation by sex steroids, but rather by paracrine mechanisms within the stem cell niche.
American Psychological Association (APA)
Schüring, A. N.& Braun, J.& Wüllner, S.& Kiesel, L.& Gotte, Martin. 2011. mRNA-Expression of ERα, ERβ, and PR in Clonal Stem Cell Cultures Obtained from Human Endometrial Biopsies. The Scientific World Journal،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-1027899
Modern Language Association (MLA)
Schüring, A. N.…[et al.]. mRNA-Expression of ERα, ERβ, and PR in Clonal Stem Cell Cultures Obtained from Human Endometrial Biopsies. The Scientific World Journal No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-1027899
American Medical Association (AMA)
Schüring, A. N.& Braun, J.& Wüllner, S.& Kiesel, L.& Gotte, Martin. mRNA-Expression of ERα, ERβ, and PR in Clonal Stem Cell Cultures Obtained from Human Endometrial Biopsies. The Scientific World Journal. 2011. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-1027899
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1027899