Elimination of Cancer Stem-Like “Side Population” Cells in Hepatoma Cell Lines by Chinese Herbal Mixture “Tien-Hsien Liquid”
Joint Authors
Chuang, Shuang-En
Yeh, Chi-Tai
Lai, Tung-Yuan
Yao, Chih-Jung
Yeh, Chuan-Feng
Lai, Gi-Ming
Lee, Liang-Ming
Chao, Wan-Ju
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-10-14
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
There are increasing pieces of evidence suggesting that the recurrence of cancer may result from a small subpopulation of cancer stem cells, which are resistant to the conventional chemotherapy and radiotherapy.
We investigated the effects of Chinese herbal mixture Tien-Hsien Liquid (THL) on the cancer stem-like side population (SP) cells isolated from human hepatoma cells.
After sorting and subsequent culture, the SP cells from Huh7 hepatoma cells appear to have higher clonogenicity and mRNA expressions of stemness genes such as SMO, ABCG2, CD133, β-catenin, and Oct-4 than those of non-SP cells.
At dose of 2 mg/mL, THL reduced the proportion of SP cells in HepG2, Hep3B, and Huh7 cells from 1.33% to 0.49%, 1.55% to 0.43%, and 1.69% to 0.27%, respectively.
The viability and colony formation of Huh7 SP cells were effectively suppressed by THL dose-dependently, accompanied with the inhibition of stemness genes, e.g., ABCG2, CD133, and SMO.
The tumorigenicity of THL-treated Huh7 SP cells in NOD/SCID mice was also diminished.
Moreover, combination with THL could synergize the effect of doxorubicin against Huh7 SP cells.
Our data indicate that THL may act as a cancer stem cell targeting therapeutics and be regarded as complementary and integrative medicine in the treatment of hepatoma.
American Psychological Association (APA)
Yao, Chih-Jung& Yeh, Chi-Tai& Lee, Liang-Ming& Chuang, Shuang-En& Yeh, Chuan-Feng& Chao, Wan-Ju…[et al.]. 2012. Elimination of Cancer Stem-Like “Side Population” Cells in Hepatoma Cell Lines by Chinese Herbal Mixture “Tien-Hsien Liquid”. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-1028428
Modern Language Association (MLA)
Yao, Chih-Jung…[et al.]. Elimination of Cancer Stem-Like “Side Population” Cells in Hepatoma Cell Lines by Chinese Herbal Mixture “Tien-Hsien Liquid”. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-1028428
American Medical Association (AMA)
Yao, Chih-Jung& Yeh, Chi-Tai& Lee, Liang-Ming& Chuang, Shuang-En& Yeh, Chuan-Feng& Chao, Wan-Ju…[et al.]. Elimination of Cancer Stem-Like “Side Population” Cells in Hepatoma Cell Lines by Chinese Herbal Mixture “Tien-Hsien Liquid”. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-1028428
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1028428