A New Experimental Polytrauma Model in Rats: Molecular Characterization of the Early Inflammatory Response
Joint Authors
Weckbach, Sebastian
Perl, Mario
Heiland, Tim
Braumüller, Sonja
Gebhard, F.
Ignatius, Anita
Flierl, Michael A.
Stahel, Philip F.
Huber-Lang, Markus S.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-29
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
The molecular mechanisms of the immune response after polytrauma are highly complex and far from fully understood.
In this paper, we characterize a new standardized polytrauma model in rats based on the early molecular inflammatory and apoptotic response.
Methods.
Male Wistar rats (250 g, 6–10/group) were anesthetized and exposed to chest trauma (ChT), closed head injury (CHI), or Tib/Fib fracture including a soft tissue trauma (Fx + STT) or to the following combination of injuries: (1) ChT; (2) ChT + Fx + STT; (3) ChT + CHI; (4) CHI; (5) polytrauma (PT = ChT + CHI + Fx + STT).
Sham-operated rats served as negative controls.
The inflammatory response was quantified at 2 hours and 4 hours after trauma by analysis of “key” inflammatory mediators, including selected cytokines and complement components, in serum and bronchoalveolar (BAL) fluid samples.
Results.
Polytraumatized (PT) rats showed a significant systemic and intrapulmonary release of cytokines, chemokines, and complement anaphylatoxins, compared to rats with isolated injuries or selected combinations of injuries.
Conclusion.
This new rat model appears to closely mimic the early immunological response of polytrauma observed in humans and may provide a valid basis for evaluation of the complex pathophysiology and future therapeutic immune modulatory approaches in experimental polytrauma.
American Psychological Association (APA)
Weckbach, Sebastian& Perl, Mario& Heiland, Tim& Braumüller, Sonja& Stahel, Philip F.& Flierl, Michael A.…[et al.]. 2012. A New Experimental Polytrauma Model in Rats: Molecular Characterization of the Early Inflammatory Response. Mediators of Inflammation،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-1029453
Modern Language Association (MLA)
Weckbach, Sebastian…[et al.]. A New Experimental Polytrauma Model in Rats: Molecular Characterization of the Early Inflammatory Response. Mediators of Inflammation No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-1029453
American Medical Association (AMA)
Weckbach, Sebastian& Perl, Mario& Heiland, Tim& Braumüller, Sonja& Stahel, Philip F.& Flierl, Michael A.…[et al.]. A New Experimental Polytrauma Model in Rats: Molecular Characterization of the Early Inflammatory Response. Mediators of Inflammation. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-1029453
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1029453