αVβ5 and CD44 Are Oxygen-Regulated Human Embryonic Stem Cell Attachment Factors
Joint Authors
Kumar, Deepak
Gupta, Saniya
Forsyth, Nicholas R.
Yang, Ying
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-26
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Human embryonic stem cells (hESCs) have great potential for clinical therapeutic use.
However, relatively little is known of the mechanisms which dictate their specificity of adhesion to substrates through adhesion proteins including integrins.
Previous observations demonstrated enhanced clonogenicity in reduced oxygen culture systems.
Here, we demonstrated via antibody blocking experiments that αVβ5 and α6 significantly promoted hESC attachment in 2% O2 only, whereas blockage of CD44 inhibited cell attachment in 21% O2 alone.
Immunofluorescence confirmed expression of αVβ5 and CD44 in both 2% O2 and 21% O2 cultured hESCs while flow cytometry revealed significantly higher αVβ5 expression in 2% O2 versus 21% O2 cultured hESCs and higher CD44 expression in 21% O2 versus 2% O2 cultured hESCs.
Adhered hESCs following blockage of αVβ5 in 2% O2 displayed a reduction in nuclear colocalisation of Oct-4 and Nanog with little effect observed in 21% O2.
Blockage of CD44 had the converse effect with dramatic reductions in nuclear colocalisation of Oct-4 and Nanog in 21% O2 cultured hESC which retained adherence, but not in 2% O2 cultured cells.
Identification of oxygen-dependent substrate attachment mechanisms in hESCs has the potential to play a role in the development of novel substrates to improve hESC attachment and culture.
American Psychological Association (APA)
Kumar, Deepak& Gupta, Saniya& Yang, Ying& Forsyth, Nicholas R.. 2013. αVβ5 and CD44 Are Oxygen-Regulated Human Embryonic Stem Cell Attachment Factors. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1030852
Modern Language Association (MLA)
Kumar, Deepak…[et al.]. αVβ5 and CD44 Are Oxygen-Regulated Human Embryonic Stem Cell Attachment Factors. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1030852
American Medical Association (AMA)
Kumar, Deepak& Gupta, Saniya& Yang, Ying& Forsyth, Nicholas R.. αVβ5 and CD44 Are Oxygen-Regulated Human Embryonic Stem Cell Attachment Factors. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1030852
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030852