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α-Ketoglutarate Accumulation Is Not Dependent on Isocitrate Dehydrogenase Activity during Tellurite Detoxification in Escherichia coli
Joint Authors
Reinoso, Claudia A.
Vásquez, Claudio C.
Appanna, Vasu D.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-25
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Tellurite is toxic to most microorganisms because of its ability to generate oxidative stress.
However, the way in which tellurite interferes with cellular processes is not fully understood to date.
In this line, it was previously shown that tellurite-exposed cells displayed reduced activity of the α-ketoglutarate dehydrogenase complex (α-KGDH), which resulted in α-ketoglutarate (α-KG) accumulation.
In this work, we assessed if α-KG accumulation in tellurite-exposed E.
coli could also result from increased isocitrate dehydrogenase (ICDH) and glutamate dehydrogenase (GDH) activities, both enzymes involved in α-KG synthesis.
Unexpectedly both activities were found to decrease in the presence of the toxicant, an observation that seems to result from the decreased transcription of icdA and gdhA genes (encoding ICDH and GDH, resp.).
Accordingly, isocitrate levels were found to increase in tellurite-exposed E.
coli.
In the presence of the toxicant, cells lacking icdA or gdhA exhibited decreased reactive oxygen species (ROS) levels and higher tellurite sensitivity as compared to the wild type strain.
Finally, a novel branch activity of ICDH as tellurite reductase is presented.
American Psychological Association (APA)
Reinoso, Claudia A.& Appanna, Vasu D.& Vásquez, Claudio C.. 2013. α-Ketoglutarate Accumulation Is Not Dependent on Isocitrate Dehydrogenase Activity during Tellurite Detoxification in Escherichia coli. BioMed Research International،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1030908
Modern Language Association (MLA)
Reinoso, Claudia A.…[et al.]. α-Ketoglutarate Accumulation Is Not Dependent on Isocitrate Dehydrogenase Activity during Tellurite Detoxification in Escherichia coli. BioMed Research International No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1030908
American Medical Association (AMA)
Reinoso, Claudia A.& Appanna, Vasu D.& Vásquez, Claudio C.. α-Ketoglutarate Accumulation Is Not Dependent on Isocitrate Dehydrogenase Activity during Tellurite Detoxification in Escherichia coli. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1030908
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030908