Characterization of the Occult Hepatitis B Virus Variants Circulating among the Blood Donors from Eastern India
Joint Authors
Datta, Sibnarayan
Chakravarty, Runu
Biswas, Avik
Panigrahi, Rajesh
Chandra, Partha Kumar
Banerjee, Arup
Sekhar, Chakraborti
Pal, Manisha
Chakraborty, Subhashish
Bhattacharya, Prasun
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-04
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
A previous study from West Bengal documented very high rate of occult HBV infection (OBI) among the HBsAg negative blood donors.
This study was aimed to characterize the OBI strains circulating among the blood donors and to estimate the risk associated with the prevailing viral variants/mutants.
Blood samples from 2195 voluntary blood donors were included in the study.
HBsAg, HBeAg, anti-HBc, and anti-HBs statuses of the samples were done by ELISA based detection.
PCR amplification and sequencing were done to determine HBV genotypes, basal core promoter (BCP), and precore (Pre-C) mutations.
Among the study samples, 268 were anti-HBc positive/HBsAg negative, among which 65 (24.25%) were HBV DNA positive.
Phylogenetic analysis revealed the presence of HBV/D (87.23%), HBV/A (8.51%), and HBV/C (4.26%) (P<0.0001).HBV/D3 (65.85%) was the significantly prevalent subgenotype over HBV/D2 (26.83%) and HBV/D1 (7.31%) (P=0.0003).
Considerable prevalence of differential BCP (1752C, 1753C, 1762T/1764A, 1753C+1762T/1764A, 1773C, and 1814C) and reverse transcriptase (rt) gene (rtI91L, rtL93P, rtS106C, rtR110G, rtN118T, rtS119T, rtY126H, rtG127W/R, rtC136R, and rtY158H) mutations was identified.
Association of specific HBV subgenotypes with OBI was interesting and needs further study.
Clinically relevant mutations were prevalent among the OBI strains which are of serious concern.
American Psychological Association (APA)
Biswas, Avik& Panigrahi, Rajesh& Chandra, Partha Kumar& Banerjee, Arup& Datta, Sibnarayan& Pal, Manisha…[et al.]. 2013. Characterization of the Occult Hepatitis B Virus Variants Circulating among the Blood Donors from Eastern India. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1032666
Modern Language Association (MLA)
Biswas, Avik…[et al.]. Characterization of the Occult Hepatitis B Virus Variants Circulating among the Blood Donors from Eastern India. The Scientific World Journal No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1032666
American Medical Association (AMA)
Biswas, Avik& Panigrahi, Rajesh& Chandra, Partha Kumar& Banerjee, Arup& Datta, Sibnarayan& Pal, Manisha…[et al.]. Characterization of the Occult Hepatitis B Virus Variants Circulating among the Blood Donors from Eastern India. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1032666
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1032666