Heparin and Liver Heparan Sulfate Can Rescue Hepatoma Cells from Topotecan Action
Joint Authors
Kudaibergenova, S.
Bocsi, József
Fullár, Alexandra
Baghy, Kornélia
Füle, Tibor
Kovalszky, Ilona
Dudas, Jozsef
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-09-07
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Topotecan (TpT) is a major inhibitory compound of topoisomerase (topo) I that plays important roles in gene transcription and cell division.
We have previously reported that heparin and heparan sulfate (HS) might be transported to the cell nucleus and they can interact with topoisomerase I.
We hypothesized that heparin and HS might interfere with the action of TpT.
To test this hypothesis we isolated topoisomerase I containing cell nuclear protein fractions from normal liver, liver cancer tissues, and hepatoma cell lines.
The enzymatic activity of these extracts was measured in the presence of heparin, liver HS, and liver cancer HS.
In addition, topo I activity, cell viability, and apoptosis of HepG2 and Hep3B cells were investigated after heparin and TpT treatments.
Liver cancer HS inhibited topo I activity in vitro.
Heparin treatment abrogated topo I enzyme activity in Hep3B cells, but not in HepG2 cells, where the basal activity was higher.
Heparin protected the two hepatoma cell lines from TpT actions and decreased the rate of TpT induced S phase block and cell death.
These results suggest that heparin and HS might interfere with the function of TpT in liver and liver cancer.
American Psychological Association (APA)
Dudas, Jozsef& Bocsi, József& Fullár, Alexandra& Baghy, Kornélia& Füle, Tibor& Kudaibergenova, S.…[et al.]. 2014. Heparin and Liver Heparan Sulfate Can Rescue Hepatoma Cells from Topotecan Action. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1034559
Modern Language Association (MLA)
Dudas, Jozsef…[et al.]. Heparin and Liver Heparan Sulfate Can Rescue Hepatoma Cells from Topotecan Action. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1034559
American Medical Association (AMA)
Dudas, Jozsef& Bocsi, József& Fullár, Alexandra& Baghy, Kornélia& Füle, Tibor& Kudaibergenova, S.…[et al.]. Heparin and Liver Heparan Sulfate Can Rescue Hepatoma Cells from Topotecan Action. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1034559
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1034559